Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes

Stefan Bálint; Satish Rao; Mónica Marro Sánchez; Veronika Huntošová; Pavol Miškovský; Dmitri Petrov

doi:10.1117/1.3369844

1 March 2010 Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes

Stefan Bálint, Satish Rao, Mónica Marro Sánchez, Veronika Huntošová, Pavol Miškovský, Dmitri Petrov

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Journal of Biomedical Optics, Vol. 15, Issue 2, 027005 (March 2010). https://doi.org/10.1117/1.3369844

Abstract

An understanding of the mechanisms of drug diffusion and uptake through cellular membranes is critical for elucidating drug action and in the development of effective drug delivery systems. We study these processes for emodin, a potential anticancer drug, in live cancer cells using surface-enhanced Raman scattering. Micrometer-sized silica beads covered by nanosized silver colloids are passively embedded into the cell and used as sensors of the drug. We demonstrate that the technique offers distinct advantages: the possibility to study the kinetics of drug diffusion through the cellular membrane toward specific cell organelles, the detection of lower drug concentrations compared to fluorescence techniques, and less damage imparted on the cell.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Stefan Bálint, Satish Rao, Mónica Marro Sánchez, Veronika Huntošová, Pavol Miškovský, and Dmitri Petrov "Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes," Journal of Biomedical Optics 15(2), 027005 (1 March 2010). https://doi.org/10.1117/1.3369844

Published: 1 March 2010

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KEYWORDS

Diffusion

Raman spectroscopy

Surface enhanced Raman spectroscopy

Metals

Luminescence

Silver

Silica

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