Nonlinear microscopy with fiber laser continuum excitation

Fredrik Svedberg; Christian Brackmann; Thomas Hellerer; Annika M. Enejder

doi:10.1117/1.3374039

1 March 2010 Nonlinear microscopy with fiber laser continuum excitation

Fredrik Svedberg, Christian Brackmann, Thomas Hellerer, Annika M. Enejder

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Journal of Biomedical Optics, Vol. 15, Issue 2, 026026 (March 2010). https://doi.org/10.1117/1.3374039

Abstract

A compact high-power fiber-based femtosecond laser system is presented for coherent anti-Stokes Raman scattering/second-harmonic generation (CARS/SHG) microscopy, and quantitatively compared with a conventional picosecond optical parametric oscillator (OPO)-based system. While the broad spectral width of the femtosecond pulses results in 2.5 times lower image contrast and limited spectral selectivity, lipid stores, myosin, and collagen filaments in living cells can clearly be identified at 60 times lower excitation powers compared to the picosecond system. Visually the images contain the same information. Together with simple operation, small footprint, and low cost, the capabilities of this high-power all-fiber-based laser system promise a more general use of nonlinear microscopy within the biosciences.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Fredrik Svedberg, Christian Brackmann, Thomas Hellerer, and Annika M. Enejder "Nonlinear microscopy with fiber laser continuum excitation," Journal of Biomedical Optics 15(2), 026026 (1 March 2010). https://doi.org/10.1117/1.3374039

Published: 1 March 2010

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KEYWORDS

Femtosecond phenomena

Microscopy

Picosecond phenomena

Fiber lasers

Laser systems engineering

Imaging systems

Second-harmonic generation

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