Errata: Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices

Bradley Edward Losavio; Vijay Iyer; Peter Saggau

doi:10.1117/1.3311562

1 January 2010 Errata: Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices

Bradley Edward Losavio, Vijay Iyer, Peter Saggau

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 1, 019801 (January 2010). https://doi.org/10.1117/1.3311562

This article [J. Biomed. Opt. 14, 064033 (2009)] was originally published online on 31 December 2009 with errors in Table 1 . The following corrections were made:

1. In column 2, row 2, the first numeral 2 underneath the square root bracket was removed;
2. In column 2, row 4, $θ_{compensated}$ was changed to $θ_{dispersion}$ . Also “gratting” was changed to “grating.”
3. In column 4, row 6, the value “627” was changed to “672.” The corrected table appears below.

In addition, in the line following Eq. (11) on page 8, the first super script −7 was corrected to −5 to read “with the parameters

λ = 7.2 \times 10^{- 5}

cm,

c = 3 \times 10^{- 5}

cm/fsec”. All versions of the article were corrected on 12 January 2010 and the article appears correctly in print.

Table 1

Theoretical spectral dispersion and compensation of TeO2 AODs. The following parameters are used: wavelength λ=720 nm; spectral bandwidth Δλ=3.68 nm; AOD aperture D=10 nm; acoustic velocity v=676 m/sec; scan range θscan=42.6 mrad; diffraction θdiffraction=0.0634 mrad; grating pitch d=150 grooves/mm; effective compensation frequency fcomp=vd2=71.1 MHz; and compensation θgrating=2Δλfcomp/v=552 μrad.

		fmin =50 MHz	f0 =70 MHz	fmax =90 MHz
2-D spectral dispersion $θ_{dispersion}$	$Δ λ \sqrt{f_{x}^{2} + f_{y}^{2}} / v$	385	539	639 μrad
Dispersed resolution N	$θ_{scan} / θ_{dispersion}$	110	79	61 spots
Spot elongation S	$θ_{dispersion} / θ_{diffraction}$	6.1×	8.5×	10.9×
2-D spectral dispersion $θ_{compensated}$	$\| θ_{dispersion} - θ_{grating} \|$	167	13.1	141 μrad
Compensated resolution $N^{'}$	$θ_{scan} / θ_{compensated}$	253	6721	302 spots
Compensated spot elongation $S^{'}$	$θ_{compensated} / θ_{diffraction}$	2.64×	0.21×	2.22×

When θcompensated<θdiffraction , the number of compensated spots becomes N′=θscan/θdiffraction , and the scanning is diffraction limited.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Bradley Edward Losavio, Vijay Iyer, and Peter Saggau "Errata: Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices," Journal of Biomedical Optics 15(1), 019801 (1 January 2010). https://doi.org/10.1117/1.3311562

Published: 1 January 2010

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