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1.IntroductionThe main function of the cardiovascular system is to provide, through perfused vascular beds, the necessities for living tissues and organs. The distributing arterial trees transport oxygen, humoral agents, and nutrients to the vital parts of the body; in the mean time, the venous trees collect metabolic wastes.1 Microcirculation, the distal functional unit of the cardiovascular system, provides exchange sites for gases, nutrients, metabolic wastes, and thermal energy between the blood and the tissues. Pathologic microcirculation reflects the breakdown of homeostasis in organisms, which ultimately leads to tissue inviability. Thus, in vivo microvascular imaging and characterization is of significant physiological, pathophysiological, and clinical importance. Well-established clinical imaging modalities such as magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and ultrasound imaging have been adopted for vascular imaging.2, 3, 4, 5, 6, 7 However, these techniques often lack either sufficient spatial resolution or satisfactory contrast (or both) to be effective for microvascular imaging.8 To bridge this gap, optical microscopy has been widely used to dissect the delicate features of the microvasculature. Intravital microscopy (IVM), the gold standard for microcirculation studies, enables quantification of vessel count, diameter, length, density, permeability, and blood flow velocity.9 Nevertheless, to observe capillaries in vivo, IVM generally requires transillumination and surgical preparation, which disturb the intrinsic microvasculature and are restricted to limited anatomical sites. Moreover, conventional IVM lacks the depth resolution that is crucial for characterizing three-dimensional (3-D) microvascular morphology. Confocal microscopy and two-photon microscopy (TPM) avoid such invasive preparation and enable volumetric visualization of the microvasculature.10, 11 However, their imaging contrasts come primarily from exogenous fluorescent agents, which—though having been very successful in laboratory research—are still facing challenges in clinical translations.12 Orthogonal polarization spectral (OPS) imaging enables intrinsic microvascular imaging;13 however, it provides only a two-dimensional (2-D) visualization of the microvasculature and lacks the measurement consistency required for chronic studies.14 Doppler optical coherence tomography (D-OCT) demonstrates the intrinsic imaging of microvascular perfusion by extracting blood flow information,15 but its resolution and sensitivity are not yet sufficient to image single capillaries. More importantly, among the mainstream techniques already mentioned, only OPS imaging can assess the functional parameter of total hemoglobin concentration (HbT), and none of them have direct access to hemoglobin oxygen saturation —another important functional parameter. To measure in vivo, the following strategies have been adopted. First, Raman spectroscopy has been integrated into IVM; however, the resonance Raman signals generated in tissue compartments other than blood could interfere with the analysis of hemoglobin.16 Second, hyperspectral imaging has been integrated into optical coherence tomography (OCT); nevertheless, it requires transillumination and provides only 2-D mapping.17 Finally, TPM has recently made exciting progress in terms of label-free imaging.18, 19 With TPM, microvascular morphology and oxygenation have been imaged in vivo without fluorescence labeling; however, quantification is not yet available due to the limited signal-to-noise ratio19 (SNR). In contrast, photoacoustic tomography (PAT) can overcome these limitations. Photoacoustics, a physical phenomenon first reported for wireless communication,20 describes the generation of acoustic waves from an object absorbing pulsed or intensity-modulated optical irradiation. With technical advances in laser sources and ultrasonic detectors, photoacoustics was investigated by the biomedical imaging community for tomographic imaging. In PAT, the target is irradiated by a short-pulsed or intensity-modulated continuous-wave (cw) laser beam, and ultrasonic waves (referred to as photoacoustic waves) are induced as a result of the transient thermoelastic expansion.21, 22 By detecting the excited photoacoustic signals, PAT can reveal the physiologically specific absorption signatures of endogenous chromophores, such as hemoglobin and melanin in vivo. More importantly, with spectroscopic measurements, PAT can quantify hemoglobin oxygenation within single vessels, providing important metabolic information about the microcirculation. A comprehensive comparison of PAT with the well-established in vivo microvascular microscopy and clinical angiography can be found in Table 1 . Table 1Comparison between PAT and well-established in vivo microvascular microscopy and clinical angiography.
CM, confocal microscopy; US, ultrasound imaging. The advantages of PAT are summarized as follows. First, endogenous optical absorption contrast enables23, 24, 25 label-free imaging of the microvasculature with a high SNR. Second, in vivo vascular imaging of small animal models can cover the length scale from a superficial capillary25 to an abdominal aorta,26 demonstrating the potential of PAT to bridge the resolution and penetration gaps between microvascular microscopy and clinical angiography. Third, spectroscopic measurement enables vessel-by-vessel mapping of blood oxygenation.27, 28 Fourth, time-resolved ultrasonic detection avoids depth scanning. Finally, working in reflection or orthogonal mode noninvasively makes this technique applicable to more anatomical sites in vivo. This paper is focused on the photoacoustic imaging and characterization of the microvasculature; however, PAT is not limited to this application. General reviews on other aspects of PAT can be found in previously published papers.21, 22, 29, 30, 31 The rest of the paper is organized as follows. First, three different embodiments of PAT are reviewed, including photoacoustic microscopy (PAM), photoacoustic computed tomography (PACT), and photoacoustic endoscopy (PAE). Then, the strategies used to characterize functional microvascular parameters—such as HbT, , and blood flow—are discussed. Afterward, some representative applications of photoacoustic microvascular imaging are introduced, including both physiological studies and clinically translatable research. The paper ends with a short discussion of several technical advances and potential prospects. 2.PAT SystemPAT has three major forms of implementation. The first one, as just mentioned, is microscopy based on 2-D raster scanning. Optical-resolution photoacoustic microscopy25, 32, 33, 34, 35, 36 (OR-PAM) and acoustic-resolution photoacoustic microscopy24, 28, 37, 38, 39 (AR-PAM) fall into this category. The second form is computed tomography based on a circular-scanning single-element ultrasonic transducer,23, 40, 41, 42 a one-dimensional (1-D) linear/curved ultrasound array,26, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54 a 2-D optical-interferometry-based acoustic sensor,55, 56, 57, 58, 59, 60 or other multiple-detector configurations, where a software- or hardware-based reconstruction algorithm is required to form an image. The third form is endoscopy,61 which has recently been advanced from 1-D sensing to 2-D imaging.62, 63 Another form of miniaturization is aimed at intravascular imaging.64, 65, 66 Table 2 compares various embodiments of PAT.24, 25, 38, 46, 60, 63, 67 In the interest of manuscript length, other novel designs68, 69, 70, 71 are not covered in detail. Table 2Comparison between different embodiments of PAT.
2.1.OR-PAMIn PAM, volumetric imaging is realized by point-scanning the dual foci of optical illumination and ultrasonic detection. To maximize the imaging sensitivity, these two foci are configured coaxially and confocally. In this case, the lateral resolution of PAM is determined by the product of the two point-spread functions (PSFs). Since capillary-level acoustic resolution requires very high ultrasonic frequency and thereby seriously limits the penetration depth, nearly diffraction-limited optical focusing is employed to achieve micrometer resolution. This technique is referred to as OR-PAM. OR-PAM, capable of resolving single red blood cells,72 was first reported for in vivo label-free capillary imaging,25 and later was refined for microhemodynamic studies34 and chronic microvascular imaging.33 As shown33 in Fig. 1 , the illumination source is a laser system consisting of a pump laser and a dye laser, which provides a wavelength-tunable output with a pulse width and a repetition rate of up to . The laser beam is reshaped by a pinhole and then focused by a microscope objective into a nearly diffraction-limited spot. An acoustic-optical beamsplitter, consisting of two right-angle prisms and a thin layer of silicone oil, is positioned under the objective lens to separate optical illumination and acoustic detection. A ultrasonic transducer is attached to the vertical side of the bottom prism. Generated photoacoustic signals are collected by an acoustic lens, redirected by the acoustic-optical beamsplitter, and received by the transducer. Through time-resolved ultrasonic detection and 2-D raster scanning along the transverse plane, complete volumetric information is recorded, and can be viewed in maximum amplitude projection (MAP) image [Fig. 2 ] or direct 3-D rendering [Fig. 2; video available in Ref. 34]. Recently, the imaging speed of OR-PAM has been significantly improved by utilizing galvanometer-based optical scanning; however, the SNR can be improved further.35 2.2.AR-PAMOR-PAM, relying on the nearly diffraction-limited optical focusing, has a penetration limit similar to that of the existing optical ballistic imaging modalities. To break through the so-called soft limit—one optical transport mean free path ( in biological tissues)—Maslov developed AR-PAM to probe, with high spatial resolution, photon absorption in the diffusive regime.37 As already mentioned, the lateral resolution of PAM is determined by the overlap of the acoustic and optical foci. Since ultrasonic scattering per unit path length in tissues is two to three orders of magnitude weaker than optical scattering,73 acoustic focusing is adopted to maintain a relatively high spatial resolution in deep tissues (up to several centimeters). Optical illumination is still weakly focused, enabling more efficient local photon energy deposition. This technique is referred to as AR-PAM. Figure 3 shows the most mature version74 of AR-PAM, called dark-field PAM. Here, a pulsed laser beam from a wavelength-tunable laser system is delivered through an optical fiber and reshaped by a conical lens to form a ring pattern. It is then weakly focused by an optical condenser lens to overlap the tight ultrasonic focus beneath the tissue. The optical illumination is solid only within the focal zone and is donut-shaped elsewhere, which greatly eliminates the surface signals. Moreover, with this dark-field configuration, an ultrasonic transducer can be placed within the dark zone, providing no interference with the optical illumination. A representative microvascular image acquired by AR-PAM is shown74 in Fig. 4 . Note that the spatial resolution, as well as the penetration depth, is highly scalable according to the ultrasonic frequency chosen.21 However, there is a trade-off between the spatial resolution and the penetration depth: higher ultrasonic frequency gives better acoustic resolution but also presents more acoustic attenuation in biological tissues. For example, a lateral resolution of and a maximum penetration of more than was achieved24, 75 with an ultrasonic center frequency of [acoustic lens numerical aperture (NA): 0.44]; a lateral resolution of and a penetration of up to was achieved38, 39 with a center frequency of (transducer /#: 1.33). When the acoustic resolution is larger than , the typical lateral resolution of human eyes at a normal observation distance, this instrument turns into an acoustic-resolution photoacoustic macroscope (AR-PAMac). 2.3.Ultrasound-Array-Based Photoacoustic Computed Tomography (UA-PACT)Although PAM offers high resolution and sensitivity, the acoustic-optical confocal configuration is somewhat sophisticated, the 2-D raster scanning is usually time-consuming, and the depth of focus is sometimes insufficient to image tissues with uneven surfaces.76 The limited depth of focus, however, can be extended using the synthetic aperture algorithm based on a virtual detector concept.38, 77 To overcome all these limitations, the entire region of interest (ROI) is simultaneously excited by a full-field optical illumination, and the photoacoustic data acquired by an ultrasound array is numerically focused to essentially everywhere in the ROI for cross-sectional scan (B-scan) or volumetric imaging without mechanical scanning. This technique is referred to as UA-PACT, although the principle of PACT can be demonstrated with a single-element ultrasonic transducer.23, 67 Figure 5 shows a representative linear-array-based PACT system.46 The pulsed laser beam delivered via a multimode optic fiber is split into two routes. The two beams are reflected and then focused by their respective cylindrical lenses. Dark-field illumination is adopted to increase the local fluence, and thus a fast PAM laser (at repetition rate) can be utilized, in combination with a 48-element linear transducer array, to achieve real-time imaging. With this configuration, a B-scan frame rate and a volumetric frame rate have been achieved.46 Besides the linear array, a curved transducer array has also been used in PACT to image the anatomical sites with curved surface contours, such as the brain or the peripheral joint. Figure 6 shows a representative curved-array-based PACT system.50 The laser beam from a wavelength-tunable laser system is expanded and homogenized to provide uniform incident fluence. A 512-element ring-shape array encircles the ROI to detect the photoacoustic signals. The detected signals are amplified and digitized before a software-based image reconstruction is performed. Since the optical illumination covers the whole ROI, which is much larger than the focal area in PAM, stronger laser pulse energy is required to maintain sufficient local fluence. In this case, a pump laser with higher pulse energy than the high-repetition-rate PAM laser is required, at the expense of the repetition rate . With this curved-array-based PACT system, a maximum imaging rate of with full tomographic resolution was achieved, which is limited by the data acquisition system. Although beyond the scope of this paper, note that Brecht recently has successfully demonstrated 3-D whole-body imaging of nude mice in vivo using a curved-array-based PAT system. 26 2.4.Optical-Detection-Based Photoacoustic Computed Tomography (OD-PACT)Although the piezoelectric ultrasound array has been widely used in PAT, its detection sensitivity falls off with decreasing element size. A higher frequency array offers higher spatial resolution at the expense of SNR. To overcome this limitation, several different optical methods have been proposed for ultrasonic detection.55, 56, 57, 58, 59, 60, 78, 79 So far, the Fabry-Pérot interferometer (FPI)-based PACT system56 (Fig. 7 ) shows the best performance among all the optical-detection methods. In this mechanism, a planar FPI is placed in contact with the tissue surface. Thus, the optical thickness of the FPI is modulated by the photoacoustic signal exiting the tissue. A focused cw probe beam is launched onto the FPI, and the local modulation in the optical thickness is encoded in the back-reflected interference signal generated by the FPI. A 2-D raster scanning of the focused probe beam will provide an ultrasonic pressure mapping on the tissue surface. A reconstruction algorithm will further recover the volumetric distribution of the tissue absorption60 (Fig. 8 ). 2.5.PAEAlthough PAM and PACT have broken through the soft limit in biological tissues, they cannot go beyond the hard limit in their present forms.21 However, deep imaging with adequate spatial resolution is highly desirable for the clinical translation of PAT, as numerous cardiovascular diseases lie in internal organs away from the skin surface. With minimally invasive endoscopy, efficient optical illumination and ultrasonic detection of interior organs can be achieved at distance from the skin surface far beyond the hard limit. A prototype of 1-D sensing PAE was reported by Viator, 61 and recently a B-mode PAE was developed.62, 63 Figure 9 shows the recently reported PAE system.62 In the endoscopic probe, the pulsed laser beam from a high-repetition-rate PAM laser is delivered by an optical fiber and emitted through the central hole in an ultrasonic transducer. A mirror, driven by a micromotor through two magnets, rotates both the optical illumination and the ultrasonic detection to accomplish a cross-sectional scan (Fig. 10 ). In the future, a spiral-scanning mechanism can be adopted from OCT endoscopy80 for volumetric imaging. 3.Photoacoustic Characterization of Functional Microvascular ParametersBesides the microvascular morphology information that can be directly extracted from a 2-D (Ref. 81) or 3-D (Ref. 82) photoacoustic image, several important functional parameters, such as HbT, , and blood flow, are also available or potentially measurable on additional imaging acquisition and data processing. These functional parameters are closely related to local energy metabolism and have been widely used in neuroscience23, 83 and disease84, 85 studies. 3.1.Quantification of Total Concentration and Oxygen Saturation of HemoglobinIn photoacoustic measurements of HbT and , we assume that in the visible spectral range, oxyhemoglobin and deoxyhemoglobin (HbR) are the dominant absorbers in blood.27 Taking advantage of the oxygenation dependence in hemoglobin absorption, PAT can use spectral measurements to quantify the relative concentrations of and HbR within single microvessels, on which the relative HbT and absolute can be computed. Based on the preceding assumption, the blood absorption coefficient can be expressed as28 where is the local blood absorption coefficient at wavelength ; and are the molar extinction coefficients of HbR and , respectively, which can be found from previously published measurements;86, 87 [HbR] and are their relative concentrations at the local position. The detected photoacoustic signal amplitude is proportional to the product of and the local optical fluence.73 Assuming that the optical fluence is wavelength independent, we can replace by in Eq. 1 and calculate [HbR] and in relative values. Afterward, the can be computed asAlthough a dual-wavelength measurement is adequate to calculate , using more wavelengths is expected to yield more accurate results.28Here, the optical fluence is assumed to be wavelength independent, which is usually an approximation because of the wavelength dependence in tissue absorption and scattering. To recover accurate values, fluence compensation is necessary. Substantive work has been undertaken on this topic; multiple numerical-model-based88, 89, 90 and experimental-measurement-based27, 28 compensation strategies have been proposed. Although the numerical model works fairly well in phantom studies, it still requires further improvements to take into account the strong heterogeneity in the structure and composition of real biological tissues. Some experimental approaches are to measure the photoacoustic spectrum of an inserted neutral absorber in vivo 28 or the wavelength-dependent light transmission through a piece of excised tissue in vitro,27 which are quite invasive and represent tentative measures. Recently, Rajian employed an optical contrast agent to enhance the performance of spectroscopic photoacoustic techniques.91 The local optical fluence can be recovered by photoacoustic measurements with and without the contrast agent in a tissue phantom. This method is noninvasive and relatively simple. In vivo clinical application is highly likely. HbT can be computed in relative values as the summation of [HbR] and . Alternatively, HbT can also be directly measured in a relative manner. In the visible and near-IR regions of the hemoglobin absorption spectra,86 there are multiple isosbestic points (390, 422, 452, 500, 530, 545, 570, 584, and ) where the molar extinction coefficients of HbR and are equal. Thus, photoacoustic signals acquired at such wavelengths reflect HbT only, regardless of the hemoglobin oxygenation level. 3.2.Photoacoustic Flow MeasurementBlood flow, in combination with the current available photoacoustic measurements of HbT, of , and of vessel diameter, can enable the quantification of tissue oxygen metabolism, which can potentially be used to visualize neuroactivity or to measure hypermetabolism—a hallmark of cancer.22 However, pulsed or color Doppler ultrasound imaging is not ideal for measuring the microvascular blood flow due to limited sensitivity and spatial resolution. D-OCT has been very successful in blood flow mapping.92, 93, 94, 95 Nevertheless, the so-called interference fringe blurring limits its maximum detectable velocity.96 Recently, Fang invented photoacoustic Doppler flowmetry97, 98 (PADF), where the undergoing photoacoustic Doppler shift due to the relative motion between the photoacoustic source and the acoustic detector is measured to reveal the traveling speed of the photoacoustic source. Flow measurements based on the observed photoacoustic Doppler effect have been performed in both clear and turbid media. Figure 11 is a schematic of the PADF system.97 The targeted optically absorbing particle with a flow speed of is excited by an intensity-modulated laser beam. The photoacoustic process is the same as that for a stationary particle. Because of the particle motion, the photoacoustic wave detected by an ultrasonic transducer has a Doppler shift expressed as97 where is the intensity-modulation frequency, is the speed of sound, and is the angle between the propagating direction of the detected photoacoustic wave and the particle traveling direction. Given an experimentally measured , the particle flow speed can be computed.As discussed in Refs. 97, 98, the power spectrum of the measured photoacoustic Doppler signal broadens with increasing flow speed. Thus, the maximum detectable velocity by PADF is limited by the SNR because the spectral amplitude will eventually approach the noise level due to the spectral broadening. The current PADF system cannot detect flow speed greater than in turbid media because of the poor SNR of the intensity-modulated photoacoustic mechanism.98, 99 To measure in vivo blood flow at a higher speed, a PADF system with higher SNR is required. Theoretically, the minimum detectable velocity (velocity sensitivity) by PADF can be as low as , which is limited by the Brownian motion;97 however, the instability in the light-intensity modulation is expected to further degrade the velocity sensitivity. The experimentally measured98 velocity sensitivity of the current PADF system is , which is adequate to detect blood flow in capillaries (the average velocity is97 a fraction of mm/s). However, a better velocity sensitivity is highly desirable to cover some cases with extremely slow capillary flows, as reported in the dermis.100, 101 Moreover, PADF can detect the flow only along the ultrasonic axis.102 To image transverse flow and slow capillary flow, Fang and Wang102 developed an M-mode photoacoustic particle imaging velocimetry based on OR-PAM. A similar configuration was previously employed by Zharov 103, 104, 105 and Galauzha 106 for counting circulating cells in vivo. Recently, Li 107 and Wei 108 reported another photoacoustic flow measurement based on the wash-in analysis of gold nanorods. In the proposed method, high-energy laser pulses at the absorption peak of the nanorod are used to induce changes in the nanorod shape; consequently, the absorption peak of the nanorods is shifted, and the photoacoustic signal is significantly reduced. On the wash-in of new gold nanorods, the photoacoustic signal is retrieved. The replenishing rate of the nanorod is highly correlated with the flow speed. Thus, by analyzing the time-intensity curve of the photoacoustic signal, researchers can recover the flow information. 4.Photoacoustic Microvascular Imaging in BiomedicineHaving the capabilities of providing high endogenous contrast, good spatial and temporal resolutions, deep tissue penetration, and important morphological and functional information, PAT holds potentially broad applications in microvascular-related physiological, pathophysiological, and clinical studies. Only a few representative applications can be covered here, in the interest of manuscript length. More applications can be found in literature.65, 109 4.1.Microhemodynamic MonitoringVasodilation and vasomotion110, 111 are two important microhemodynamic activities in tissue oxygen regulation; however, the underlying mechanism of vasomotion remains elusive.110 Taking advantage of the high spatial and temporal resolutions of OR-PAM, Hu recently explored vasomotion and vasodilation in response to tissue oxygen variation.34 A representative microvascular network in a nude mouse ear was imaged by a dual-wavelength OR-PAM measurement under systemic normoxia (Fig. 12 ). According to the mapping, a B-scan position was selected for microhemodynamic monitoring, which contained an arteriole-venule pair [A1 and V1 in Fig. 12]. During the monitoring session, the physiological state of the mouse was switched between systemic hyperoxia and hypoxia. Vasomotion and vasodilation were clearly exhibited, respectively, by the oscillation and the expansion in vessel diameters [Figs. 13 and 13 ]. To provide quantitative analysis, the vessel diameter was estimated by the full width at half maximum (FWHM) value of the blood vessel signal in each B-scan [Fig. 13]. Data analysis [Figs. 13 and 13] suggested that arteriole A1 had a more significant vasomotion than did V1 under hyperoxia but with a similar oscillation frequency of (cpm), which is in good agreement with the observation from a previous invasive study.112 By isolating the vasodilation effect, an increase of in the arteriolar diameter and in the venular diameter under hypoxia was observed [Fig. 13], which agrees with a scanning laser fluorescent ophthalmoscopy study.113 The response time of vasodilation to systemic hypoxia is estimated to be . Some other hemodynamics—such as blood oxygenation variation in the mouse brain cortex, vessel response to photodynamic therapy, and blood/hemolymph sedimentation—have also been studied by PAT, as can be referenced in previously published papers.114, 115, 116, 117 4.2.Functional Chronic ImagingChronic imaging of the microcirculation enables direct visualization of long-term microhemodynamics, opening a window for tracing disease progression (for example, tumor angiogenesis24, 118, 119), neural plasticity, and functional recovery from pathological states. Recently, the capability of OR-PAM for functional chronic imaging was demonstrated by monitoring the wound-healing process in murine microvasculature in vivo.33 A microvascular network in a mouse ear was randomly picked out, and the central part was treated under a focused cw laser beam for to create a microvascular wound. The ROI was imaged, both before [Fig. 14 ] and immediately after the cw laser treatment [Fig. 14], as well as in the subsequent [Figs. 14(c-1) to 14(c-12)], using both OR-PAM and a commercial transmission-mode optical microscope. The results clearly show a four-step wound-healing process: (1) vessel regression and hemostasis [Fig. 14]; (2) inflammation induced vasodilation [Figs. 14(c-1) to 14(c-5)], and hypoxia facilitated angiogenesis [Figs. 14 and 14(c-1) to 14(c-5)]; (3) ingrowth of new capillaries to restore the microcirculation [Fig. 14(c-3)]; and (4) morphological and functional recovery [Fig. 14(c-12)]. This observation is confirmed by known physiology.120 4.3.Tumor-Vascular InteractionsTumor progression depends on a number of sequential steps, including initial tumor-vascular interactions, recruitment of blood vessels, and tumor cells interacting with the surrounding microenvironment. The failure of a tumor to recruit new vasculature or to reorganize the existing surrounding vasculature results in a nonangiogenic and nonprogressing stable tumor.121 Thus, functional imaging of tumor-vascular interactions is of great clinical importance. Recently, spectroscopic photoacoustic tomography (SPAT) was applied in combination with molecular biomarkers to study the interrelationships between hemodynamics and tumor progression.85 Human U87 glioblastoma tumor cells were implanted stereotactically into young adult immunocompromised nude mice. One week after inoculation, IRDye800-c(KRGDf) was systematically administered through the tail vein to visualize newly formed tumor microvessels. Figure 15 shows the tumor angiogenesis image acquired by SPAT after the injection of IRDye800-c(KRGDf). Tumor hypoxia was also imaged by SPAT [Fig. 15]. Quantitative analysis reveals that the level in the tumor vasculature is more heterogeneous than that in the normal vessels, and the average in the tumor vasculature is at least 13% lower than that in the normal vasculature. With finer spatial resolution, PAT can directly visualize tumor angiogenesis.24 Figure 16 shows the AR-PAM imaging of a rat vascular network at three different time points: before, after, and after the subcutaneous inoculation of tumor cells. Tumor vasculature exhibits greater vessel complexity and higher density than the surrounding healthy tissue.24 4.4.Neurovascular CouplingAdvances in brain imaging facilitate the fundamental understanding of cognitive phenomena and neurological diseases. However, the traditional procedure of using microelectrodes is quite invasive, thereby limiting the number of recordings and discouraging chronic study. Alternatively, downstream hemodynamics originating from the brain microcirculation has been recorded to retrace brain functions.83 PACT was applied to visualize the response in the brain cortex of rats to whisker stimulation.23 HbT with and without whisker stimulation are recorded sequentially at an isosbestic optical wavelength. Functional signal—the difference in photoacoustic signal strength recorded with and without stimulation—is expected to be from the stimulation-induced hemodynamic change. The experimental results show that vascular patterns in the activated regions match the distribution of functional signals well, indicating that the functional signals detected by PACT did result from hemodynamic changes in the blood vessels of the superficial cortex. Histological evaluation also shows a good colocalization between the whisker-barrel cortex and the activated regions in the PACT images. Note that OR-PAM has successfully demonstrated brain microvascular imaging down to single capillaries through intact mouse skulls in vivo.32 Functional brain mapping at micrometer resolution is anticipated. 5.Discussion and PerspectivesTaking advantage of the highly scalable spatial resolution and penetration depth, and of the unique optical absorption contrast, PAT will no doubt find its position in fundamental physiological research and clinical care, especially in the cardiovascular field. Several technical advances, as follows, might make it serve better. 5.1.System Refinement Toward Clinical ApplicationsPAT is safe for clinical applications as it generally delivers optical fluence well within the American National Standards Institute (ANSI) safety standards.21, 24, 75 AR-PAM has imaged the subcutaneous microvasculature in a human palm, showing24 a high contrast-to-noise ratio of . Several pilot clinical studies have been reported in imaging port-wine stains122, 123, 124 (PWS), HbT (Refs. 125, 126) and (Ref. 127). However, technical improvement is required to facilitate an effective clinical translation. For internal organ (such as gastrointestinal tract and prostate) imaging, PAE is an ideal tool. Recent progress in PAE is exciting;62, 63 nevertheless, its size must be further reduced to be compatible with generic endoscopes, and its spatial and temporal resolutions require improvements. For cutaneous applications, such as skin cancer detection and cutaneous vascular lesion diagnosis, a multiscale, handheld photoacoustic probe is required, which would enable users to select an optimal trade-off between the spatial resolution and the penetration depth for different clinical applications. Multimodality imaging, providing complementary information for more accurate diagnosis, is always favorable. PAT combined with confocal microscopy, TPM, OCT (Ref. 128), ultrasound imaging,47, 129, 130 or MRI (Ref. 131) will provide fruitful anatomical, functional, and molecular information. 5.2.SNR and Contrast EnhancementIn PAT, the high optical absorption of hemoglobin in the visible spectral region provides strong photoacoustic signals and high SNR at an ANSI-approved optical fluence level. However, the high absorption also prevents photons from penetrating into deeper regions. When the near-IR window is used for deep penetration, the photoacoustic signal from the microvasculature is compromised and typically requires averaging to enhance the SNR and contrast.21 Current strategies, such as the synthetic aperture focusing technique77, 132 (SAFT), the virtual point detection mechanism,133, 134 the adaptive beam forming method,135 and the exogenous vascular contrast agent136, 137, 138 have made remarkable progress. However, further exploration is required to escape from this dilemma. 5.3.Fluence CompensationAs discussed in Sec. 3.1, PAT is capable of imaging in microvasculature by comparing the experimentally measured photoacoustic spectrum with the known absorption spectra of HbR and . Using a spectroscopic analysis, the relative concentrations of HbR and can be recovered. However, spectroscopic problems such as this are more challenging in photoacoustic imaging mainly because PAT detects specific optical absorption—proportional to the product of the absorption coefficient and the local optical fluence—rather than the absorption coefficient.139 Accurate fluence compensation will promote PAT from qualitative or quasiquantitative characterization to a higher level, offering more robust quantitative analysis toward clinical applications. AcknowledgmentsThe authors appreciate Dr. Lynnea Brumbaugh’s close reading of the manuscript. The authors thank Drs. Konstantin Maslov, Bin Rao, and Christopher Favazza for helpful discussion. This work is sponsored in part by National Institutes of Health Grants R01 EB000712, R01 NS46214 (Bioengineering Research Partnerships), R01 EB008085, and U54 CA136398 (Network for Translational Research). L.V.W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work. ReferencesJ. K.-J. Li, Dynamics of the Vascular System, World Scientific, Singapore
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