Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Lingfeng Yu; Samarendra Kumar Mohanty; Gangjun Liu; Suzanne Genc; Zhongping Chen; Michael W. Berns

doi:10.1117/1.2997375

1 September 2008 Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Kumar Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, Michael W. Berns

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Journal of Biomedical Optics, Vol. 13, Issue 5, 050508 (September 2008). https://doi.org/10.1117/1.2997375

Abstract

The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Lingfeng Yu, Samarendra Kumar Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, and Michael W. Berns "Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery," Journal of Biomedical Optics 13(5), 050508 (1 September 2008). https://doi.org/10.1117/1.2997375

Published: 1 September 2008

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