Use of scaling relations to extract intrinsic fluorescence lifetime of targets embedded in turbid media

Victor V. Chernomordik; Moinuddin Hassan; Franck Amyot; Jason D. Riley; Amir H. Gandjbakhche

doi:10.1117/1.2904660

1 March 2008 Use of scaling relations to extract intrinsic fluorescence lifetime of targets embedded in turbid media

Victor V. Chernomordik, Moinuddin Hassan, Franck Amyot, Jason D. Riley, Amir H. Gandjbakhche

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Journal of Biomedical Optics, Vol. 13, Issue 2, 024025 (March 2008). https://doi.org/10.1117/1.2904660

Abstract

We present a novel method for estimating the intrinsic fluorescence lifetime of deeply embedded localized fluorophores. It is based on scaling relations, characteristic for turbid media. The approach is experimentally substantiated by successfully reconstructing lifetimes for targets at depths up to 14.5 mm. A derived correction factor was determined from the product of the transport-corrected scattering coefficient μ_s^′ and the index of refraction nr. In addition, data from an array of detectors (≥2) can be used to estimate μ_s^′ n_r. The suggested algorithm is a promising tool for diagnostic fluorescence, since lifetime can be a sensitive indicator of the fluorophore environment.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Victor V. Chernomordik, Moinuddin Hassan, Franck Amyot, Jason D. Riley, and Amir H. Gandjbakhche "Use of scaling relations to extract intrinsic fluorescence lifetime of targets embedded in turbid media," Journal of Biomedical Optics 13(2), 024025 (1 March 2008). https://doi.org/10.1117/1.2904660

Published: 1 March 2008

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