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1 March 2008 Measurements of light scattering in an integrated microfluidic waveguide cytometer
Xuan-Tao Su, Kirat Singh, Clarence E. Capjack, Jiri Petracek, Christopher J. Backhouse, Wojciech Rozmus
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Journal of Biomedical Optics, Vol. 13, Issue 2, 024024 (March 2008). https://doi.org/10.1117/1.2909670
Abstract
An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.
©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Xuan-Tao Su, Kirat Singh, Clarence E. Capjack, Jiri Petracek, Christopher J. Backhouse, and Wojciech Rozmus "Measurements of light scattering in an integrated microfluidic waveguide cytometer," Journal of Biomedical Optics 13(2), 024024 (1 March 2008). https://doi.org/10.1117/1.2909670
Published: 1 March 2008
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KEYWORDS
Light scattering
Waveguides
Charge-coupled devices
Microfluidics
Mie scattering
Scatter measurement
Optical testing
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