Quantitative phase microscopy through differential interference imaging

Sharon V. King; Ariel R. Libertun; Rafael Piestun; Carol J. Cogswell; Chrysanthe Preza

doi:10.1117/1.2907328

1 March 2008 Quantitative phase microscopy through differential interference imaging

Sharon V. King, Ariel R. Libertun, Rafael Piestun, Carol J. Cogswell, Chrysanthe Preza

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Journal of Biomedical Optics, Vol. 13, Issue 2, 024020 (March 2008). https://doi.org/10.1117/1.2907328

Abstract

An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Sharon V. King, Ariel R. Libertun, Rafael Piestun, Carol J. Cogswell, and Chrysanthe Preza "Quantitative phase microscopy through differential interference imaging," Journal of Biomedical Optics 13(2), 024020 (1 March 2008). https://doi.org/10.1117/1.2907328

Published: 1 March 2008

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