Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy

Thuc T. Le; Ingeborg M. Langohr; Matthew J. Locker; Michael Sturek; Ji-Xin Cheng

doi:10.1117/1.2795437

1 September 2007 Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy

Thuc T. Le, Ingeborg M. Langohr, Matthew J. Locker, Michael Sturek, Ji-Xin Cheng

Author Affiliations +

Journal of Biomedical Optics, Vol. 12, Issue 5, 054007 (September 2007). https://doi.org/10.1117/1.2795437

Abstract

Arterial tissues collected from Ossabaw swine bearing metabolic syndrome-induced cardiovascular plaques are characterized by multimodal nonlinear optical microscopy that allows coherent anti-Stokes Raman scattering, second-harmonic generation, and two-photon excitation fluorescence imaging on the same platform. Significant components of arterial walls and atherosclerotic lesions, including endothelial cells, extracellular lipid droplets, lipid-rich cells, low-density lipoprotein aggregates, collagen, and elastin are imaged without any labeling. Emission spectra of these components are obtained by nonlinear optical microspectrometry. The nonlinear optical contrast is compared with histology of the same sample. Multimodal nonlinear optical imaging of plaque composition also allows identification of atherosclerotic regions that are vulnerable to rupture risk. The demonstrated capability of nonlinear optical microscopy for label-free molecular imaging of atherosclerotic lesions with 3-D submicrometric resolution suggests its potential application to the diagnosis of atherosclerotic plaques, determination of their rupture risk, and design of individualized drug therapy based on plaque composition.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Thuc T. Le, Ingeborg M. Langohr, Matthew J. Locker, Michael Sturek, and Ji-Xin Cheng "Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy," Journal of Biomedical Optics 12(5), 054007 (1 September 2007). https://doi.org/10.1117/1.2795437

Published: 1 September 2007

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KEYWORDS

Collagen

Second-harmonic generation

Arteries

Microscopy

Nonlinear optics

Molecular imaging

CARS tomography

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