Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo

Sourav Kumar Kar; Ananda Kumar; Fabao Gao; Bensheng Qiu; Xiangcan Zhan; Xiaoming Yang

doi:10.1117/1.2209559

1 May 2006 Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo

Sourav Kumar Kar, Ananda Kumar, Fabao Gao, Bensheng Qiu, Xiangcan Zhan, Xiaoming Yang

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Journal of Biomedical Optics, Vol. 11, Issue 3, 034008 (May 2006). https://doi.org/10.1117/1.2209559

Abstract

This study develops a percutaneous optical imaging system for tracking fluorescent reporter gene expression in vasculatures. We build a percutaneous optical imaging system that primarily comprised a 1.5-mm, semi-rigid, two-port optical probe. The performance of the optical probe is first tested in vitro with cell phantoms, and then the feasibility of the percutaneous optical imaging system is validated in vivo in eight femoral artery segments of two pigs. The green fluorescent protein (GFP) gene is locally delivered into four arterial segments, while saline is delivered to the four contralateral arterial segments as controls. The targeted arteries are localized using color Doppler, and thereafter the optical probe is positioned to the target arterial segments under ultrasound guidance. Optical imaging captures are obtained using different exposure times from 10 to 60 s. Subsequently, the GFP- and saline-targeted arteries are harvested for fluorescent microscopy confirmation. The percutaneous optical probe is successfully positioned at a distance approximately 2 mm from the targets in all eight arteries. The in vivo imaging shows higher average signal intensity in GFP-treated arteries than in saline-treated arteries. This study demonstrates the potential using the percutaneous optical imaging system to monitor, in vivo, reporter gene expression from vasculatures.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Sourav Kumar Kar, Ananda Kumar, Fabao Gao, Bensheng Qiu, Xiangcan Zhan, and Xiaoming Yang "Percutaneous optical imaging system to track reporter gene expression from vasculatures in vivo," Journal of Biomedical Optics 11(3), 034008 (1 May 2006). https://doi.org/10.1117/1.2209559

Published: 1 May 2006

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