Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source

Shuo Tang; Tatiana B. Krasieva; Zhongping Chen; Bruce Jason Tromberg

doi:10.1117/1.2193428

1 March 2006 Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source

Shuo Tang, Tatiana B. Krasieva, Zhongping Chen, Bruce Jason Tromberg

Author Affiliations +

Journal of Biomedical Optics, Vol. 11, Issue 2, 020502 (March 2006). https://doi.org/10.1117/1.2193428

Abstract

A 12-fs broadband (100-nm) source is used to combine multiphoton microscopy (MPM) and optical coherence tomography (OCT) in a single platform. An ultrafast Ti:sapphire laser simultaneously provides short pulses necessary for efficient MPM excitation and the broad bandwidth required for high-resolution OCT. Using 0.3-µm microspheres and a 63×, 0.95 numerical aperture objective, we demonstrate that MPM and OCT channels are coregistered with lateral resolution of approximately 0.5 µm and axial resolution of approximately 1.5 µm. Preliminary studies of a 3-D organotypic epithelial tissue model show that multiphoton images of fluorescence and second harmonic signals are derived from cellular and extracellular matrix structures, respectively, while OCT images are generated from scattering interfaces due to tissue variations in refractive index. The combined MPM/OCT microscope is capable of providing simultaneous functional and structural information from cells and extracellular matrix and is potentially a powerful tool for studying biological processes in thick tissues.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Shuo Tang, Tatiana B. Krasieva, Zhongping Chen, and Bruce Jason Tromberg "Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source," Journal of Biomedical Optics 11(2), 020502 (1 March 2006). https://doi.org/10.1117/1.2193428

Published: 1 March 2006

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KEYWORDS

Optical coherence tomography

Second-harmonic generation

Tissues

Objectives

Multiphoton microscopy

Signal detection

Collagen

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