Imaging intracellular neurotransmitters with vibrational microscopy using isotopologues

Gabriel F. Dorlhiac; Bryce Manifold; Markita Landry; Aaron Streets

doi:10.1117/12.3017344

20 June 2024 Imaging intracellular neurotransmitters with vibrational microscopy using isotopologues

Gabriel F. Dorlhiac, Bryce Manifold, Markita Landry, Aaron Streets

Proceedings Volume PC13007, Neurophotonics II; PC1300705 (2024) https://doi.org/10.1117/12.3017344
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

We present a new approach for imaging intracellular neurotransmitter molecules with stimulated Raman scattering microscopy. We leverage the isolated vibrational peaks of carbon-deuterium bonds to observe these neurotransmitters directly and quantitatively. By using deuterated versions of neurotransmitters, we minimize perturbation to neurochemical activity with respect to previously demonstrated fluorescence-based methods. We show direct imaging of deuterated dopamine and GABA uptake and release dynamics in PC12 chromafin cells, and in primary hippocampal neurons, respectively. Specifically, we show that stimulation of neurotransmitter release results in a 20-50% intracellular neurotransmitter concentration reduction, with the ability to observe inter- and intracellular variation in vesicular neurotransmitter release. Taken together, our data suggest that neurotransmitter isotopologues can serve as a generic, commercially-available, non-perturbative, and biocompatible method to image neurotransmitters that are chemically homologous to their native counterparts.

Conference Presentation

Citation Download Citation

Gabriel F. Dorlhiac, Bryce Manifold, Markita Landry, and Aaron Streets "Imaging intracellular neurotransmitters with vibrational microscopy using isotopologues", Proc. SPIE PC13007, Neurophotonics II, PC1300705 (20 June 2024); https://doi.org/10.1117/12.3017344

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available