Exploring a new Fourier transform-based hyperspectral imaging technology and its application in microscopy

Marta Ghirardello; Lorenzo Vinco; Dario Polli; Alex Barker; Antonio Perri; Fabrizio Preda

doi:10.1117/12.3000896

12 March 2024 Exploring a new Fourier transform-based hyperspectral imaging technology and its application in microscopy

Marta Ghirardello, Lorenzo Vinco, Dario Polli, Alex Barker, Antonio Perri, Fabrizio Preda

Author Affiliations +

Proceedings Volume PC12893, Photonic Instrumentation Engineering XI; PC128930G (2024) https://doi.org/10.1117/12.3000896
Event: SPIE OPTO, 2024, San Francisco, California, United States

Abstract

Hyperspectral imaging is rapidly advancing and transforming industries such as agriculture, medicine, and defense. The introduction of HERA, a new hyperspectral camera, is a notable development in this field. Utilizing a Fourier-transform approach, HERA consists of a monochromatic camera, camera lens, and an innovative common-path birefringent interferometer. By scanning the interferometer's position and capturing a sequence of monochromatic images, the hyperspectral data-cube is acquired without any camera or sample movement. This approach offers advantages like high light throughput, signal-to-noise ratio, and wavelength accuracy. HERA's exceptional light throughput enables high-quality data even in low-illuminance conditions and fluorescence studies. Additionally, its flexibility and stability allow for integration with commercial microscopes, expanding the applications of hyperspectral imaging to the microscopy field. This work focuses on showcasing HERA's significant applications in microbiology.

Conference Presentation

Citation Download Citation

Marta Ghirardello, Lorenzo Vinco, Dario Polli, Alex Barker, Antonio Perri, and Fabrizio Preda "Exploring a new Fourier transform-based hyperspectral imaging technology and its application in microscopy", Proc. SPIE PC12893, Photonic Instrumentation Engineering XI, PC128930G (12 March 2024); https://doi.org/10.1117/12.3000896

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