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13 March 2024 Rapid fabrication of a bioimaging micro-chip using two-photon polymerization
Behjat S. Kariman, Alessandra Nardini, Marco Grassi, Mario Marini, Claudio Conci, Margaux Bouzin, Maddalena Collini, Manuela T. Raimondi, Giuseppe Chirico, Roberto Osellame, Giulio Cerullo, Rebeca Martínez Vázquez
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Proceedings Volume PC12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV; PC1287503 (2024) https://doi.org/10.1117/12.3002218
Event: SPIE LASE, 2024, San Francisco, California, United States
Abstract
Deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. In this framework we propose a miniaturized in-vivo imaging window composed of high dioptric power microlenses coupled to micro-scaffold, which were fabricated by two-photon polymerization (2PP) in the biocompatible photoresist SZ2080. We adopt a single-irradiation strategy for the fabrication of the whole structure: We first irradiate the micro-scaffold on the bottom side of the chip and afterwards the microlenses on the top. For the microlenses we adopted a hybrid approach by combining the 2PP of the micro lenses surface with a subsequent UV crosslinking of the inner volume. We explored different lenses profiles (plano-convex and parabolic) with variable parameters like diameter and focal lengths, to optimize the imaging characteristics. We envisage that these imaging windows will open the way to direct and continuous optical inspection of biological processes in vivo.
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Behjat S. Kariman, Alessandra Nardini, Marco Grassi, Mario Marini, Claudio Conci, Margaux Bouzin, Maddalena Collini, Manuela T. Raimondi, Giuseppe Chirico, Roberto Osellame, Giulio Cerullo, and Rebeca Martínez Vázquez "Rapid fabrication of a bioimaging micro-chip using two-photon polymerization", Proc. SPIE PC12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV, PC1287503 (13 March 2024); https://doi.org/10.1117/12.3002218
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KEYWORDS
Two photon polymerization
Fabrication
Biological imaging
In vivo imaging
Microlens
Confocal microscopy
Lenses
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