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13 March 2024 Intracellular heat conduction and heat-induced temperature change measurements with mid-infrared photothermal optical diffraction tomography
Keiichiro Toda, Masaharu Takarada, Genki Ishigane, Hiroyuki Shimada, Venkata Ramaiah Badarla, Kohki Okabe, Takuro Ideguchi
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285513 (2024) https://doi.org/10.1117/12.3003105
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Fluorescent thermometry has reported noteworthy intracellular temperature gradients, suggesting the possibility of thermal regulation of cellular functions. However, heat conduction calculations assuming an aqueous intracellular environment contradict these findings. To resolve this contradiction, we visualized intracellular heat conduction using label-free mid-infrared photothermal optical diffraction tomography and obtained thermal diffusivity similar to that of water. Furthermore, we found a discrepancy in heat-induced temperature changes measured with label-free and fluorescent thermometry. This suggests that fluorescent thermometers may sense other factors, including the system’s internal energy, in addition to the temperature at a local equilibrium state.
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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Keiichiro Toda, Masaharu Takarada, Genki Ishigane, Hiroyuki Shimada, Venkata Ramaiah Badarla, Kohki Okabe, and Takuro Ideguchi "Intracellular heat conduction and heat-induced temperature change measurements with mid-infrared photothermal optical diffraction tomography", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285513 (13 March 2024); https://doi.org/10.1117/12.3003105
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KEYWORDS
Temperature metrology
Diffraction
Mid-IR
Optical tomography
Thermometry
Nanoprobes
Thermography
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