Photothermal mid-infrared microscopy

Gwendylan Turner; Daniel I Herman; Markus Mangold; Aleksandr Razumtcev; Ziyi Cao; Jiayue Rong; Charles A. Bouman; Gregery Buzzard; Garth J. Simpson

doi:10.1117/12.3003026

13 March 2024 Photothermal mid-infrared microscopy

Gwendylan Turner, Daniel I Herman, Markus Mangold, Aleksandr Razumtcev, Ziyi Cao, Jiayue Rong, Charles A. Bouman, Gregery Buzzard, Garth J. Simpson

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285510 (2024) https://doi.org/10.1117/12.3003026
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

An approach is described and evaluated for spectrally parallel hyperspectral mid-infrared imaging with optical spatial resolution. Dual-comb mid-infrared spectroscopy using a commercial QCL DCS system (IRis-core, IRsweep) enables acquisition of infrared spectra at high speed (<1 millisecond) through generation of optical beat patterns and radio-frequency detection. The high-speed nature of the spectral acquisition is shown to support spectral mapping in microscopy measurements. Direct detection of the transmitted infrared beam yields excellent spectral information, but the long infrared wavelength imposes low diffraction-limited spatial resolution. Use of fluorescence-detected photothermal infrared (FPTIR) imaging provides high spatial resolution tied directly to the integrated IR absorption. Computational imaging using a multi-agent consensus equilibrium (MACE) approach combines the high spatial resolution of FPTIR and the high spectral information of dual-comb infrared transmission in a single optimized equilibrium hyperspectral data cube.

Conference Presentation

Citation Download Citation

Gwendylan Turner, Daniel I Herman, Markus Mangold, Aleksandr Razumtcev, Ziyi Cao, Jiayue Rong, Charles A. Bouman, Gregery Buzzard, and Garth J. Simpson "Photothermal mid-infrared microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285510 (13 March 2024); https://doi.org/10.1117/12.3003026

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