Autofluorescence imaging reveals metabolic impacts of substrate properties on stem cell-derived cardiomyocytes

Danielle E. Desa; Margot J. Amitrano; William L. Murphy; Melissa C. Skala

doi:10.1117/12.3003077

13 March 2024 Autofluorescence imaging reveals metabolic impacts of substrate properties on stem cell-derived cardiomyocytes

Danielle E. Desa, Margot J. Amitrano, William L. Murphy, Melissa C. Skala

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285408 (2024) https://doi.org/10.1117/12.3003077
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Induced pluripotent stem cells (iPSC) can be differentiated into cardiomyocytes (CM) for disease modeling and drug screening. Batch variability in differentiated cells frequently occurs due to heterogeneity in commercial culture substrates. We performed optical metabolic imaging (OMI) on synthetic hydrogels of varying stiffnesses and compositions and observed expected metabolic shifts as iPSCs differentiated. OMI revealed metabolic differences between cells cultured on heterogenous commercial substrates and synthetic hydrogels and can be used to monitor cell sensitivity to the microenvironment. These data demonstrate OMI is a powerful tool for identifying iPSC differentiation and maturation conditions, crucial in stem cell manufacturing.

Conference Presentation

Citation Download Citation

Danielle E. Desa, Margot J. Amitrano, William L. Murphy, and Melissa C. Skala "Autofluorescence imaging reveals metabolic impacts of substrate properties on stem cell-derived cardiomyocytes", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285408 (13 March 2024); https://doi.org/10.1117/12.3003077

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