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13 March 2024 Fluorescence microscopy through scattering layers by a virtual reflection-matrix
Gil Weinberg, Elad Sunray, Ori Katz
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Proceedings Volume PC12851, Adaptive Optics and Wavefront Control for Biological Systems X; PC1285109 (2024) https://doi.org/10.1117/12.3008707
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Optical-resolution fluorescence imaging in complex samples is challenging due to random light scattering, with significant implications across multiple fields. Here, we adapt computational reflection-matrix techniques developed for scattering compensation in phase-sensitive coherent imaging, to incoherent fluorescence imaging that solely relies on intensity detection. Moreover, we numerically and experimentally demonstrate that the adoption of random illuminations can substantially decrease the number of measurements necessary for effective reflection-matrix solutions.
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Gil Weinberg, Elad Sunray, and Ori Katz "Fluorescence microscopy through scattering layers by a virtual reflection-matrix", Proc. SPIE PC12851, Adaptive Optics and Wavefront Control for Biological Systems X, PC1285109 (13 March 2024); https://doi.org/10.1117/12.3008707
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KEYWORDS
Matrices
Fluorescence imaging
Fluorescence
Light scattering
Scattering
Reflection
Coherence imaging
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