Two-photon bi-functional refractive Index and fluorescence microscopy (2P-BRIEF)

Yucheng Li; Yi Xue

doi:10.1117/12.3001958

13 March 2024 Two-photon bi-functional refractive Index and fluorescence microscopy (2P-BRIEF)

Yucheng Li , Yi Xue

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480A (2024) https://doi.org/10.1117/12.3001958
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Understanding the dynamic interaction between fluorescence-labeled and label-free structures is important. The two types of signals are conventionally collected by fluorescence and phase microscopy, respectively. Recently, we developed a one-photon bi-functional microscopy system for simultaneous imaging of fluorescence and refractive index. However, it cannot reconstruct the refractive index of highly scattering samples with dense fluorophores. To solve this challenge, we develop new two-photon multimodal microscopy that reconstructs the 3D refractive index and fluorescence from multiple two-photon fluorescence images with a multi-slice model. We validated this method using a sample consisting of a mixture of fluorescence and glass beads.

Conference Presentation

Citation Download Citation

Yucheng Li and Yi Xue "Two-photon bi-functional refractive Index and fluorescence microscopy (2P-BRIEF)", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480A (13 March 2024); https://doi.org/10.1117/12.3001958

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