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13 March 2024 Myth FLIM
Hauke Studier, Julius Heitz
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284707 (2024) https://doi.org/10.1117/12.3002687
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Fluorescence Lifetime Imaging (FLIM) was introduced in 1993 by the company Becker & Hickl and is no longer a myth. However, some myths have arisen around the topic of FLIM over time. Since the Mythbusters have not yet dealt with this topic, I would like to take up and classify some of these myths in this presentation. This includes questions like, what significantly influences the measurement time in my FLIM experiment or also in which kind of FLIM experiment does a high temporal precision help me. As an example for a FLIM hot topic, I will also show metabolic imaging. Finally, I will briefly introduce new Becker & Hickl hardware and what it can do for you.
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Hauke Studier and Julius Heitz "Myth FLIM", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284707 (13 March 2024); https://doi.org/10.1117/12.3002687
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KEYWORDS
Fluorescence lifetime imaging
Biological samples
Quantum experiments
Quantum limits
Time correlated single photon counting
Fluorescence
Fluorophores
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