High power and energy tunable lasers for 2-photon imaging

Marco Arrigoni; Jonas Berzinš; Lukas Kontenis; Mantvydas Mikulis

doi:10.1117/12.3014131

13 March 2024 High power and energy tunable lasers for 2-photon imaging

Marco Arrigoni, Jonas Berzinš, Lukas Kontenis, Mantvydas Mikulis

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284706 (2024) https://doi.org/10.1117/12.3014131
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

2-Photon imaging has dramatically expanded its reach in neuroscience-related applications thanks to the development of genetically encoded functional probes that report cellular activity and activates/silence individual neurons (optogenetics). The combination of 3D spatial resolution afforded by 2-photon imaging and the proliferation of advanced scanning and scanless methodologies enable powerful parallel functional imaging of thousands of individually selected neurons. Faster and parallel imaging of large cell populations requires femtosecond lasers with higher average power and energy, but also improved pulse-to-pulse stability all along the required tuning ranges. Light Conversion has pioneered the development of femtosecond wavelength-tunable devices as well as of high-energy and -power Ytterbium lasers ad amplifiers. In this presentation we highlight how these developments are powering advanced imaging techniques using reporting and optogenetic probes.

Conference Presentation

Citation Download Citation

Marco Arrigoni, Jonas Berzinš, Lukas Kontenis, and Mantvydas Mikulis "High power and energy tunable lasers for 2-photon imaging", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284706 (13 March 2024); https://doi.org/10.1117/12.3014131

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available