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13 March 2024 Real-time heart rate analysis of cardiac organoid using optical hybrid digital holographic microscope
Ik Hwan Kwon, Tae Geol Lee, Sang-Won Lee
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Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC1284608 (2024) https://doi.org/10.1117/12.3002878
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We present a novel technique that uses hybrid digital holography to observe three-dimensional morphological changes in cardiac organoids and measure heart rates simultaneously. By reconstructing the real-time spatial topology, we capture dynamic morphological changes in three-dimensional cardiac organoids, especially in the central region where traditional optical imaging methods are limited. We analyze the correlation between the phase analysis results of digital holography and the corresponding electronic signal amplitude with heartbeat data. Fluorescent modes can also be used to investigate heart rate, ROS activation, and organic cell activation. In addition, dark field mode allows you to simultaneously evaluate the distribution of nanoparticles about 100 nm in size on the heart surface and their effect on the heart rate. Our work demonstrates the potential of hybrid digital holography as an innovative optical analysis method in heart and tissue analysis, providing valuable insights into the relationship between cardiac tissue dynamics and heart rate
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Ik Hwan Kwon, Tae Geol Lee, and Sang-Won Lee "Real-time heart rate analysis of cardiac organoid using optical hybrid digital holographic microscope", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC1284608 (13 March 2024); https://doi.org/10.1117/12.3002878
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KEYWORDS
Digital holography
Heart
Holography
Hybrid optics
Microscopes
Tissues
3D metrology
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