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We present mesoscale imaging of nanoparticle-labeled cells in 3D culture with photothermal optical coherence tomography (PT-OCT). Cells labeled with gold nanorods (AuNRs) are encapsulated in agarose-based gel for PT-OCT with 660-nm laser. We show a binary PT contrast for single cell detection without causing cell death. We also demonstrate 3D mapping of AuNR-labeled cells within optically scattering media by imaging through a layer of polystyrene beads mimicking highly scattering tissue and through a layer of cells without AuNRs. This method has an imaging scale that complements the other major cell imaging techniques for studying live cells in 3D culture.
Jingyu Sun,Tianqi Fang,Hongjun Wang, andShang Wang
"Imaging of nanoparticle-labeled live cells in 3D culture with photothermal optical coherence tomography (OCT)", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC1284109 (13 March 2024); https://doi.org/10.1117/12.3006228
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Jingyu Sun, Tianqi Fang, Hongjun Wang, Shang Wang, "Imaging of nanoparticle-labeled live cells in 3D culture with photothermal optical coherence tomography (OCT)," Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC1284109 (13 March 2024); https://doi.org/10.1117/12.3006228