Affordable optofluidic add-on for continuous light sheet imaging of larval and adult c. elegans

Faraz Rahimpouresfahani; Pouya Rezai; Nima Tabatabaei

doi:10.1117/12.3001808

13 March 2024 Affordable optofluidic add-on for continuous light sheet imaging of larval and adult c. elegans

Faraz Rahimpouresfahani, Pouya Rezai, Nima Tabatabaei

Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC128370C (2024) https://doi.org/10.1117/12.3001808
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

This paper introduces an optimized and affordable optofluidic add-on device for continuous and rapid light sheet imaging of C. elegans. Platform utilizes an optofluidic chip with integrated PDMS microlens to generate a light-sheet and rapidly images worms flowing in a microchannel via a regular microscope. Validation results on control and 6-OHDA-exposed worms demonstrates the ability of platform in qualitative and quantitative imaging of neuronal degenerations at different ages. The developed affordable platform enables a conventional fluorescent microscope to offer high-content and high-throughput light-sheet images of C. elegans populations which is invaluable to aging, neurodegeneration, and drug discovery studies.

Conference Presentation

Citation Download Citation

Faraz Rahimpouresfahani, Pouya Rezai, and Nima Tabatabaei "Affordable optofluidic add-on for continuous light sheet imaging of larval and adult c. elegans", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC128370C (13 March 2024); https://doi.org/10.1117/12.3001808

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