Inactivation of bacteria using ultrashort UVC laser pulses operating at 206nm or 222nm wavelengths: a pilot study

Asta Valanciute

doi:10.1117/12.3001079

13 March 2024 Inactivation of bacteria using ultrashort UVC laser pulses operating at 206nm or 222nm wavelengths: a pilot study

Asta Valanciute

Author Affiliations +

Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC128220O (2024) https://doi.org/10.1117/12.3001079
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The purpose of this study was to elucidate the bactericidal effect of femtosecond pulsed laser operating at 206 nm or 222 nm wavelength. We experimentally investigated bactericidal capacity against Gram-negative and Gram-positive pathogenic bacteria (Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Methicillin resistant Staphylococcus aureus). The settled bacteria culture on a Luria-Bertani agar plate was irradiated with 206 nm or 222 nm UVC light with irradiation dose of 1, 5 or 25 mJ/cm2. We used a pulse repetition rate (PRR) of 10 kHz during our irradiation. We find that the pulsed far-UVC light with wavelength of 222 nm and 206 nm was significantly germicidal on Gram-negative and Gram-positive bacteria. Both wavelengths were as efficient in killing bugs as 255 nm UVC. Direct absorption of 206 nm or 222 nm UVC irradiation led to a generation of reactive oxygen species.

Conference Presentation

Citation Download Citation

Asta Valanciute "Inactivation of bacteria using ultrashort UVC laser pulses operating at 206nm or 222nm wavelengths: a pilot study", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC128220O (13 March 2024); https://doi.org/10.1117/12.3001079

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