Laser bioprinting of cells and tumor organoids for organ-on-chip applications

Evina Elezoglou; Maria Chliara; Marianneza Chatzipetrou; ‪Symeon Papazoglou; Katerina Tsilingiri; Apostolos Klinakis; Ioanna Zergioti

doi:10.1117/12.2648068

17 March 2023 Laser bioprinting of cells and tumor organoids for organ-on-chip applications

Evina Elezoglou, Maria Chliara, Marianneza Chatzipetrou, ‪Symeon Papazoglou, Katerina Tsilingiri, Apostolos Klinakis, Ioanna Zergioti

Author Affiliations +

Proceedings Volume PC12411, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIII; PC1241107 (2023) https://doi.org/10.1117/12.2648068
Event: SPIE LASE, 2023, San Francisco, California, United States

Abstract

Bioprinting is a rapidly expanding additive manufacturing process, for the development of complex biostructures such as tissue-like structures, able to imitate native tissue functions. Among the most commonly used bioprinting techniques, Laser Induced Forward Transfer (LIFT) offers the highest degree of spatial resolution (minimum feature of <10 μm) and post-printing cell viability while it has also been used for the immobilization of biomaterials on a variety of substrates. Furthermore, this study will present the control of the depth of cell deposition within the ECM by tuning the laser printing parameters and the organoid formation of the laser printed cells, already from Day 1 after bioprinting. Finally, the application of LIFT technique in Organ on Chip applications will be presented.

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Citation Download Citation

Evina Elezoglou, Maria Chliara, Marianneza Chatzipetrou, ‪Symeon Papazoglou, Katerina Tsilingiri, Apostolos Klinakis, and Ioanna Zergioti "Laser bioprinting of cells and tumor organoids for organ-on-chip applications", Proc. SPIE PC12411, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIII, PC1241107 (17 March 2023); https://doi.org/10.1117/12.2648068

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