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15 March 2023 Broadband coherent Raman microscopy and deep learning for high-speed imaging of biological samples (Conference Presentation)
Dario Polli, Federico Vernuccio, Alejandro De la Cadena, Arianna Bresci, Chiara Ceconello, Francesco Manetti, Salvatore Sorrentino, Subir Das, Renzo Vanna, Giulio N. Cerullo
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920M (2023) https://doi.org/10.1117/12.2648054
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We present several approaches to high-speed broadband stimulated Raman scattering (SRS) and broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy and microscopy for label-free chemical identification of molecules through their intrinsic vibrational spectrum. (1) A 32-channels lock-in amplifier for parallel detection of broadband SRS spectra. (2) A broadband CARS microscope covering the entire fingerprint with down to 1-ms pixel dwell time, based on a low-repetition-rate amplified laser allowing white-light supercontinuum generation in bulk media. (3) Artificial-intelligence-based methods to enhance the signal-to-noise ratio and remove the non-resonant background signal from CARS spectra. (4) Video-rate wide-field CARS microscopy over the fingerprint region.
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Dario Polli, Federico Vernuccio, Alejandro De la Cadena, Arianna Bresci, Chiara Ceconello, Francesco Manetti, Salvatore Sorrentino, Subir Das, Renzo Vanna, and Giulio N. Cerullo "Broadband coherent Raman microscopy and deep learning for high-speed imaging of biological samples (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920M (15 March 2023); https://doi.org/10.1117/12.2648054
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KEYWORDS
Microscopy
Raman spectroscopy
High speed imaging
Raman scattering
Signal detection
Molecules
Optical amplifiers
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