Single virus detection by wide-field interferometric defocus-enhanced mid-infrared photothermal microscopy (Conference Presentation)

Qing Xia; Ji-Xin Cheng; John H. Connor; Zhongyue Guo; Haonan Zong; Scott Seitz; Le Wang; Celalettin Yurdakul

doi:10.1117/12.2647635

15 March 2023 Single virus detection by wide-field interferometric defocus-enhanced mid-infrared photothermal microscopy (Conference Presentation)

Qing Xia, Ji-Xin Cheng, John H. Connor, Zhongyue Guo, Haonan Zong, Scott Seitz, Le Wang, Celalettin Yurdakul

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239205 (2023) https://doi.org/10.1117/12.2647635
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Label-free spectroscopic detection of single viruses provides component analysis of virus strains. Current methods suffer from low throughput and weak signal contrast of individual virions. Here, we report a wide-field interferometric defocus-enhanced mid-infrared photothermal (WIDE-MIP) microscopy for high-throughput fingerprinting of single viruses. Comprehensive theoretical frameworks for WIDE-MIP signal is developed and experimentally validated by acquiring defocused interferometric and photothermal images. WIDE-MIP not only provides the amide I and amide II vibrations in viral proteins, but also reveals the unique IR signature of thymine and uracil residues vibration in DNA vaccinia viruses and RNA vesicular stomatitis viruses, respectively.

Conference Presentation

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Qing Xia, Ji-Xin Cheng, John H. Connor, Zhongyue Guo, Haonan Zong, Scott Seitz, Le Wang, and Celalettin Yurdakul "Single virus detection by wide-field interferometric defocus-enhanced mid-infrared photothermal microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239205 (15 March 2023); https://doi.org/10.1117/12.2647635

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KEYWORDS

Interferometry

Viruses

Microscopy

Mid-IR

Chemical analysis

Spectroscopy

Infrared signatures

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