Multi-focal structured illumination microscopy for deeper penetration superresolution imaging

Junle Qu

doi:10.1117/12.2656855

30 March 2023 Multi-focal structured illumination microscopy for deeper penetration superresolution imaging

Junle Qu

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC1239001 (2023) https://doi.org/10.1117/12.2656855
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We developed an adaptive optics based multifocal structured illumination microscopy system (AO-MSIM) for deep penetration, superresolution imaging. A spatial light modulator was used to generate a multi-focus excitation lattice, and multi-focus structured illumination imaging is achieved by stacking the phase of the grating. Based on the detected wavefront information, a spatial light modulator and a deformable mirror were used to correct the aberrations of excitation and emission paths respectively. By using pixel relocation and deconvolution image reconstruction algorithm, spatial resolution of ~150nm was achieved at an imaging depth of 500μm. The applications of AO-MSIM technology in imaging thick brain tissue samples will be demonstrated.

Conference Presentation

Citation Download Citation

Junle Qu "Multi-focal structured illumination microscopy for deeper penetration superresolution imaging", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239001 (30 March 2023); https://doi.org/10.1117/12.2656855

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