1Institut de la Vision (France) 2Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France) 3Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France) 4Institut Langevin (France)
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We present the first dynamic full field optical coherence tomography (D-FFOCT) module which can be easily integrated into a commercial microscope. Benefitting from the optical standardization of commercial microscopy, we demonstrate three-dimensional live imaging experiments over time periods of minutes to hours to days thanks to the use of an incubator, able to maintain cell culture conditions. We show timelapse high resolution live images of retinal explants and organoids in disease modeling applications.
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Salvatore Azzollini, Tual Monfort, Olivier Thouvenin, Kate F. Grieve, "Disease modeling applications of dynamic full field optical coherence tomography module in retinal studies," Proc. SPIE PC12378, Dynamics and Fluctuations in Biomedical Photonics XX, PC1237803 (7 March 2023); https://doi.org/10.1117/12.2648331