A topographic Raman imaging approach for surgical margin assessment

Alexander Czaja; Alice Jiang; Olga Eremina; Cristina Zavaleta

doi:10.1117/12.2649201

17 March 2023 A topographic Raman imaging approach for surgical margin assessment

Alexander Czaja, Alice Jiang, Olga Eremina, Cristina Zavaleta

Proceedings Volume PC12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX; PC1236106 (2023) https://doi.org/10.1117/12.2649201
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Negative surgical margins can be difficult to confirm intraoperatively. We propose a workflow of immunostaining and surface imaging of fresh excised tissue using highly sensitive and spectrally separable SERS nanoparticles as the targeted contrast agent. The adaptive focus capabilities of an advanced Raman instrument, combined with our rotational accessory tool for exposing each surface of the stained specimen to the objective lens, enables topographic mapping of the entire excised specimen’s surface. Detailed surface renderings color-encoded according to unmixed SERS nanoparticle abundances show a path forward for high-content, interactive surgical margin assessment.

Conference Presentation

Citation Download Citation

Alexander Czaja, Alice Jiang, Olga Eremina, and Cristina Zavaleta "A topographic Raman imaging approach for surgical margin assessment", Proc. SPIE PC12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX, PC1236106 (17 March 2023); https://doi.org/10.1117/12.2649201

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