Chemical imaging of free fatty acid production heterogeneity in live microbes by stimulated Raman scattering

Haonan Lin; Nathan Tague; Jean-Baptiste Lugagne; Deeya Burman; Wilson W. Wong; Mary J. Dunlop; Ji-Xin Cheng

doi:10.1117/12.2607965

2 March 2022 Chemical imaging of free fatty acid production heterogeneity in live microbes by stimulated Raman scattering

Haonan Lin, Nathan Tague, Jean-Baptiste Lugagne, Deeya Burman, Wilson W. Wong, Mary J. Dunlop, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197317 (2022) https://doi.org/10.1117/12.2607965
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Engineered microbes can produce valuable chemicals but often require extensive optimization to reach sufficient production yields. Current quantitation methods such as gas chromatography-mass spectrometry are based on ensemble measurements that ignore the genetic or phenotypic variation among cells. Here, we use hyperspectral stimulated Raman scattering (hsSRS) to directly image free fatty acid synthesis in metabolically engineered Escherichia coli and uncover substantial heterogeneity among and within colonies. Using optimized laser exposures, we perform longitudinal SRS imaging to link the dynamics of biofuel synthesis with cell growth. Further, we develop an analytical protocol to enable mass spectrometry-like chemical specificity estimates of fatty acids.

Conference Presentation

Citation Download Citation

Haonan Lin, Nathan Tague, Jean-Baptiste Lugagne, Deeya Burman, Wilson W. Wong, Mary J. Dunlop, and Ji-Xin Cheng "Chemical imaging of free fatty acid production heterogeneity in live microbes by stimulated Raman scattering", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197317 (2 March 2022); https://doi.org/10.1117/12.2607965

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