Nanosecond-resolution photothermal dynamic imaging via MHz digitization and match filtering

Jiaze Yin; Lu Lan; Yi Zhang; Hongli Ni; Yuying Tan; Meng Zhang; Yeran Bai; Ji-Xin Cheng

doi:10.1117/12.2610500

2 March 2022 Nanosecond-resolution photothermal dynamic imaging via MHz digitization and match filtering

Jiaze Yin, Lu Lan, Yi Zhang, Hongli Ni, Yuying Tan, Meng Zhang, Yeran Bai, Ji-Xin Cheng

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197313 (2022) https://doi.org/10.1117/12.2610500
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Photothermal microscopy has enabled highly sensitive label-free imaging of absorbers, from metallic nanoparticles to chemical bonds. Photothermal signals are conventionally detected via modulation of excitation beam and demodulation of probe beam using lock-in amplifier. While convenient, the wealth of thermal dynamics is not revealed. Here, we present a lock-in free, mid-infrared photothermal dynamic imaging (PDI) system by MHz digitization and match filtering at harmonics of modulation frequency. Thermal-dynamic information is acquired at nanosecond resolution within single pulse excitation. Our method not only increases the imaging speed by two orders of magnitude but also obtains four-fold enhancement of signal-to-noise ratio over lock-in counterpart, enabling high-throughput metabolism analysis at single-cell level. Moreover, by harnessing the thermal decay difference between water and biomolecules, water background is effectively separated in mid-infrared PDI of living cells. This ability to nondestructively probe chemically specific photothermal dynamics offers a valuable tool to characterize biological and material specimens.

Conference Presentation

Citation Download Citation

Jiaze Yin, Lu Lan, Yi Zhang, Hongli Ni, Yuying Tan, Meng Zhang, Yeran Bai, and Ji-Xin Cheng "Nanosecond-resolution photothermal dynamic imaging via MHz digitization and match filtering", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197313 (2 March 2022); https://doi.org/10.1117/12.2610500

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