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2 March 2022 Raman image-activated cell sorting and beyond
Keisuke Goda, Kotaro Hiramatsu
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Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730K (2022) https://doi.org/10.1117/12.2608054
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We present Raman image-activated cell sorting (RIACS), a technology that performs real-time coherent Raman image-based sorting of single live cells with a throughput of up to ~100 cells per second. Specifically, the fast image acquisition of the RIACS is enabled by multicolor stimulated Raman scattering (SRS) imaging based on a pulse-pair-resolved wavelength-switchable laser. To show the broad utility of the RIACS, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.
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Keisuke Goda and Kotaro Hiramatsu "Raman image-activated cell sorting and beyond", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730K (2 March 2022); https://doi.org/10.1117/12.2608054
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KEYWORDS
Raman spectroscopy
Image acquisition
Laser scattering
Raman scattering
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