Dynamically heterogeneous plasma membrane is poised for initiation of receptor-mediated mast cell signaling

Barbara A. Baird; Nirmalya Bag; David Holowka

doi:10.1117/12.2613064

7 March 2022 Dynamically heterogeneous plasma membrane is poised for initiation of receptor-mediated mast cell signaling

Barbara A. Baird, Nirmalya Bag, David Holowka

Author Affiliations +

Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC1196506 (2022) https://doi.org/10.1117/12.2613064
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Cell surface receptors distinguish specific stimuli from biological noise, and underlying mechanisms depend on the organization of plasma membrane components. We exploited the statistical robustness of multiplexed Imaging Fluorescence Correlation Spectroscopy (ImFCS), to evaluate subtle diffusion differences of key components in the early stage of mast cell signaling by measuring their diffusion in live cells: FcεRI receptor for immunoglobulin E (IgE), tyrosine kinase Lyn anchored to the membrane inner leaflet, and transmembrane phosphatase PTPα. Our results confirm that the nanoscale distribution of signaling proteins is coordinately rearranged after the receptor is stimulated, such that changes in relatively weak interactions serve to transmit the signal across the membrane.

Conference Presentation

Citation Download Citation

Barbara A. Baird, Nirmalya Bag, and David Holowka "Dynamically heterogeneous plasma membrane is poised for initiation of receptor-mediated mast cell signaling", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC1196506 (7 March 2022); https://doi.org/10.1117/12.2613064

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