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7 March 2022 Photon budget analysis for label-free quantitative multiphoton microscopy
Rishyashring R. Iyer, Janet E. Sorrells, Geng Wang, Lingxiao Yang, Haohua Tu, Stephen A Boppart
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Proceedings Volume PC11951, Design and Quality for Biomedical Technologies XV; PC119510I (2022) https://doi.org/10.1117/12.2609392
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
The goal towards developing faster and minimally damaging quantitative MPM involves keeping the exposure time per pixel as low as possible. Both the excitation and detection parameters affect the number of measurements required for quantification and therefore, the overall efficiency of the setup. We present our estimation for the minimum number of photons needed and our ability to resolve them to quantify label-free MPM of NAD(P)H and FAD using their intensity, fluorescence lifetime, and optical redox ratio. We not only utilize these results to guide the imaging parameters for in vitro and ex vivo studies, but also to normalize the results from different setups.
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Rishyashring R. Iyer, Janet E. Sorrells, Geng Wang, Lingxiao Yang, Haohua Tu, and Stephen A Boppart "Photon budget analysis for label-free quantitative multiphoton microscopy", Proc. SPIE PC11951, Design and Quality for Biomedical Technologies XV, PC119510I (7 March 2022); https://doi.org/10.1117/12.2609392
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KEYWORDS
Multiphoton microscopy
Photonic microstructures
Biological research
Luminescence
In vitro testing
Photodetectors
Sensors
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