CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC11947 > Article
Presentation
7 March 2022 Spatially targeted non-viral optical delivery of opsin-encoding genes into primate retina
Biraj Mahato, Sanghoon Kim, Houssam Al-Saad, Michael Carlson, Subrata Batabyal, Adnan Dibas, Samarendra K. Mohanty
Author Affiliations +
Proceedings Volume PC11947, Optogenetics and Optical Manipulation 2022; PC1194709 (2022) https://doi.org/10.1117/12.2615466
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Degeneration of photoreceptors, retinal cells including retinal pigment epithelium, and retinal ganglion cells characterizes visual disorders involving retinal dysfunction. The efficient and targeted gene delivery into retinal cells is critical for treatment of a variety of visual disorders. Here, we report use of an OCT guided, spatially targeted, near-infrared laser microirradiation platform to successfully deliver genes encoding ambient-light activatable multi-characteristic opsin (MCO) to retinal cells in non-human primates. The efficacy of gene delivery was evaluated by fundus imaging of the reporter (mCherry) fluorescence, and immunohistochemistry. Functional improvement was measured by red-ERG which was attributed to MCO’s broadband activation spectrum.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Biraj Mahato, Sanghoon Kim, Houssam Al-Saad, Michael Carlson, Subrata Batabyal, Adnan Dibas, and Samarendra K. Mohanty "Spatially targeted non-viral optical delivery of opsin-encoding genes into primate retina", Proc. SPIE PC11947, Optogenetics and Optical Manipulation 2022, PC1194709 (7 March 2022); https://doi.org/10.1117/12.2615466
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Retina
Continuous wave operation
Near infrared
Optical coherence tomography
Visualization
Molecules
Target designation
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top