Polarization-correlation optical microscopy of anisotropic biological layers

A. G. Ushenko; A. V. Dubolazov; V. A. Ushenko; Yu. A. Ushenko; M. Yu. Sakhnovskiy; V. N. Balazyuk; O. Khukhlina; K. Viligorska; A. Bykov; A. Doronin; I. Meglinski

doi:10.1117/12.2237926

28 September 2016 Polarization-correlation optical microscopy of anisotropic biological layers

A. G. Ushenko, A. V. Dubolazov, V. A. Ushenko, Yu. A. Ushenko, M. Yu. Sakhnovskiy, V. N. Balazyuk, O. Khukhlina, K. Viligorska, A. Bykov, A. Doronin, I. Meglinski

Author Affiliations +

Proceedings Volume 9971, Applications of Digital Image Processing XXXIX; 99712C (2016) https://doi.org/10.1117/12.2237926
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter – complex degree of mutual anisotropy – is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states – dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.

Citation Download Citation

A. G. Ushenko, A. V. Dubolazov, V. A. Ushenko, Yu. A. Ushenko, M. Yu. Sakhnovskiy, V. N. Balazyuk, O. Khukhlina, K. Viligorska, A. Bykov, A. Doronin, and I. Meglinski "Polarization-correlation optical microscopy of anisotropic biological layers", Proc. SPIE 9971, Applications of Digital Image Processing XXXIX, 99712C (28 September 2016); https://doi.org/10.1117/12.2237926

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available