Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy

Marcelina Cardoso Dos Santos; Cyrille Vézy; Rodolphe Jaffiol

doi:10.1117/12.2208429

9 March 2016 Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy

Marcelina Cardoso Dos Santos, Cyrille Vézy, Rodolphe Jaffiol

Proceedings Volume 9719, Biophysics, Biology, and Biophotonics: the Crossroads; 97190I (2016) https://doi.org/10.1117/12.2208429
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We propose a novel fluorescence microscopy technique to study the adhesion of Giant Unilamellar Vesicles. Their adhesion is assessed through the normalization of Total Internal Reflection Fluorescence (TIRF) images by epi-fluorescence ones. This can be achieved by using a motorized rotatable mirror to switch from epi-fluorescence to TIRF. This method allows us to study the vesicle adhesion from ultra-weak to strong membrane surface interactions and to measure the absolute distance between the vesicles and various chemically functionalized glass substrates at the nanoscale.

Conference Presentation

Citation Download Citation

Marcelina Cardoso Dos Santos, Cyrille Vézy, and Rodolphe Jaffiol "Vesicle adhesion depicted at nanoscale with normalized total internal reflection fluorescence microscopy", Proc. SPIE 9719, Biophysics, Biology, and Biophotonics: the Crossroads, 97190I (9 March 2016); https://doi.org/10.1117/12.2208429

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