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26 May 2016 Resonant artefacts in broadband CARS microspectroscopy (Conference Presentation)
Bradley Littleton, Thomas Kavanagh, David R. Richards
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Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 97120L (2016) https://doi.org/10.1117/12.2213620
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
Full analysis of signal contributions in broadband CARS shows that the broadband fields give rise to significant resonant spectral artefacts, in addition to the non-resonant background (NRB). These modify vibrational line amplitudes such that broadband CARS does not give accurate concentration information. We have used a spectral interferometry method (Spectral Interferometric Polarisation CARS) to examine the resonant background contributions directly, and find that with certain conditions on the Stokes spectrum resonant artefacts can be avoided in the C-H stretch spectral region, but not in the fingerprint region. A preprocessing step, however, permits fully quantitative B-CARS measurements.
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Bradley Littleton, Thomas Kavanagh, and David R. Richards "Resonant artefacts in broadband CARS microspectroscopy (Conference Presentation)", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120L (26 May 2016); https://doi.org/10.1117/12.2213620
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KEYWORDS
Imaging spectroscopy
Interferometry
Polarization
Signal analyzers
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