Universal lab-on-a-chip platform for complex, perfused 3D cell cultures

F. Sonntag; F. Schmieder; J. Ströbel; S. Grünzner; M. Busek; K. Günther; T. Steege; C. Polk; U. Klotzbach

doi:10.1117/12.2218606

21 March 2016 Universal lab-on-a-chip platform for complex, perfused 3D cell cultures

F. Sonntag, F. Schmieder, J. Ströbel, S. Grünzner, M. Busek, K. Günther, T. Steege, C. Polk, U. Klotzbach

Proceedings Volume 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV; 970516 (2016) https://doi.org/10.1117/12.2218606
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The miniaturization, rapid prototyping and automation of lab-on-a-chip technology play nowadays a very important role. Lab-on-a-chip technology is successfully implemented not only for environmental analysis and medical diagnostics, but also as replacement of animals used for the testing of substances in the pharmaceutical and cosmetics industries. For that purpose the Fraunhofer IWS and partners developed a lab-on-a-chip platform for perfused cell-based assays in the last years, which includes different micropumps, valves, channels, reservoirs and customized cell culture modules. This technology is already implemented for the characterization of different human cell cultures and organoids, like skin, liver, endothelium, hair follicle and nephron. The advanced universal lab-on-a-chip platform for complex, perfused 3D cell cultures is divided into a multilayer basic chip with integrated micropump and application-specific 3D printed cell culture modules. Moreover a technology for surface modification of the printed cell culture modules by laser micro structuring and a complex and flexibly programmable controlling device based on an embedded Linux system was developed. A universal lab-on-a-chip platform with an optional oxygenator and a cell culture module for cubic scaffolds as well as first cell culture experiments within the cell culture device will be presented. The module is designed for direct interaction with robotic dispenser systems. This offers the opportunity to combine direct organ printing of cells and scaffolds with the microfluidic cell culture module. The characterization of the developed system was done by means of Micro-Particle Image Velocimetry (μPIV) and an optical oxygen measuring system.

Conference Presentation

Citation Download Citation

F. Sonntag, F. Schmieder, J. Ströbel, S. Grünzner, M. Busek, K. Günther, T. Steege, C. Polk, and U. Klotzbach "Universal lab-on-a-chip platform for complex, perfused 3D cell cultures", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970516 (21 March 2016); https://doi.org/10.1117/12.2218606

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available