The component validation of direct diode 488nm lasers in BD Accuri C6 flow cytometers

Wei P. Chen; Ningyi D. Luo

doi:10.1117/12.2213432

18 March 2016 Component validation of direct diode 488nm lasers in BD Accuri C6 flow cytometers

Wei P. Chen, Ningyi D. Luo

Proceedings Volume 9700, Design and Quality for Biomedical Technologies IX; 97000E (2016) https://doi.org/10.1117/12.2213432
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The 488nm laser is the most important excitation light source of flow cytometry. The indirect diode (frequency-doubled diode) 488nm lasers are used in the excitation of Becton Dickinson (BD) Accuri^TM C6. For using cost effective lasers, we have validated direct diode 488nm lasers as the replacement component of frequency-doubled diode laser. BD Bioscience issued the protocols to cover wavelength, power, noise, and polarization at the operation temperature range of cytometer. Pavilion Integration Corporation (PIC) tested 6 samples as the component validation of direct diode 488nm lasers based on the protocols from BD Biosciences. BD Bioscience also tested one of laser samples to further validate the test results of power, noise, and polarization from PIC.

Conference Presentation

Citation Download Citation

Wei P. Chen and Ningyi D. Luo "Component validation of direct diode 488nm lasers in BD Accuri C6 flow cytometers", Proc. SPIE 9700, Design and Quality for Biomedical Technologies IX, 97000E (18 March 2016); https://doi.org/10.1117/12.2213432

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available