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3 May 2016 Portable point-of-care blood analysis system for global health (Conference Presentation)
James J. Dou, James Stewart Aitchison, Lu Chen, Rakesh Nayyar
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Proceedings Volume 9699, Optics and Biophotonics in Low-Resource Settings II; 96990M (2016) https://doi.org/10.1117/12.2212666
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
In this paper we present a portable blood analysis system based on a disposable cartridge and hand-held reader. The platform can perform all the sample preparation, detection and waste collection required to complete a clinical test. In order to demonstrate the utility of this approach a CD4 T cell enumeration was carried out. A handheld, point-of-care CD4 T cell system was developed based on this system. In particular we will describe a pneumatic, active pumping method to control the on-chip fluidic actuation. Reagents for the CD4 T cell counting assay were dried on a reagent plug to eliminate the need for cold chain storage when used in the field. A micromixer based on the active fluidic actuation was designed to complete sample staining with fluorescent dyes that was dried on the reagent plugs. A novel image detection and analysis algorithm was developed to detect and track the flight of target particles and cells during each analysis. The handheld, point-of-care CD4 testing system was benchmarked against clinical cytometer. The experimental results demonstrated experimental results were closely matched with the flow cytometry. The same platform can be further expanded into a bead-array detection system where other types of biomolecules such as proteins can be detected using the same detection system.
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James J. Dou, James Stewart Aitchison, Lu Chen, and Rakesh Nayyar "Portable point-of-care blood analysis system for global health (Conference Presentation)", Proc. SPIE 9699, Optics and Biophotonics in Low-Resource Settings II, 96990M (3 May 2016); https://doi.org/10.1117/12.2212666
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KEYWORDS
Point-of-care devices
Blood
Algorithm development
Image analysis
Microfluidics
Detection and tracking algorithms
Detector development
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