Reflectance confocal microscopy of red blood cells: simulation and experiment (Conference Presentation)

Adel Zeidan; Daniella Yeheskely-Hayon; Limor Minai; Dvir Yelin

doi:10.1117/12.2209027

27 April 2016 Reflectance confocal microscopy of red blood cells: simulation and experiment (Conference Presentation)

Adel Zeidan, Daniella Yeheskely-Hayon, Limor Minai, Dvir Yelin

Proceedings Volume 9691, Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III; 96910N (2016) https://doi.org/10.1117/12.2209027
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The properties of red blood cells are a remarkable indicator of the body's physiological condition; their density could indicate anemia or polycythemia, their absorption spectrum correlates with blood oxygenation, and their morphology is highly sensitive to various pathologic states including iron deficiency, ovalocytosis, and sickle cell disease. Therefore, measuring the morphology of red blood cells is important for clinical diagnosis, providing valuable indications on a patient’s health. In this work, we simulated the appearance of normal red blood cells under a reflectance confocal microscope and discovered unique relations between the cells’ morphological parameters and the resulting characteristic interference patterns. The simulation results showed good agreement with in vitro reflectance confocal images of red blood cells, acquired using spectrally encoded flow cytometry (SEFC) that imaged the cells during linear flow and without artificial staining. By matching the simulated patterns to the SEFC images of the cells, the cells’ three-dimensional shapes were evaluated and their volumes were calculated. Potential applications include measurement of the mean corpuscular volume, cell morphological abnormalities, cell stiffness under mechanical stimuli, and the detection of various hematological diseases.

Conference Presentation

Citation Download Citation

Adel Zeidan, Daniella Yeheskely-Hayon, Limor Minai, and Dvir Yelin "Reflectance confocal microscopy of red blood cells: simulation and experiment (Conference Presentation)", Proc. SPIE 9691, Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III, 96910N (27 April 2016); https://doi.org/10.1117/12.2209027

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Blood

Confocal microscopy

Reflectivity

3D image processing

Absorption

Flow cytometry

In vitro testing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years