Label free detection of optogenetically stimulated cellular activity by low coherence interferometry
(Conference Presentation)

Sarmishtha Satpathy; Subrata Batabyal; Digant P. Dave; Samarendra K Mohanty

doi:10.1117/12.2216404

26 April 2016 Label free detection of optogenetically stimulated cellular activity by low coherence interferometry (Conference Presentation)

Sarmishtha Satpathy, Subrata Batabyal, Digant P. Dave, Samarendra K Mohanty

Author Affiliations +

Proceedings Volume 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation; 96902D (2016) https://doi.org/10.1117/12.2216404
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Detecting cellular activity in sub-millisecond timescale and micrometer resolution without using invasive means has been a long standing goal in the study of cellular networks. Here, we have employed phase sensitive low coherence interferometry for detecting optogenetically stimulated activity of cells. Nanoscale changes in optical path length (due to change in refractive index and changes in cell thickness) occur when cells are activated, which we aim to detect by phase sensitive low coherence interferometry. A low coherence interferometry and patch-clamp electrophysiology systems were integrated with an inverted fluorescence microscope. Blue laser beam was coupled to the electrophysiology-interferometric detection system for optogenetic stimulation. The phase-sensitive measurements were carried out on Channelrhodopsin-2 sensitized cells (identified by YFP fluorescence) as well as control cells in reflection mode for different intensities and exposures of optogenetic stimulation beam. This method offers good temporal and spatial resolution without using exogenous labeling. Results of studies on all optical stimulation and detection of cellular activity will be presented. Interpretation of the optical activity signals will be discussed in context with changes in cell physiology during stimulation. We will also discuss the potential sources of various artifacts in optical/electrical detection of cellular activity during optical stimulation.

Conference Presentation

Citation Download Citation

Sarmishtha Satpathy, Subrata Batabyal, Digant P. Dave, and Samarendra K Mohanty "Label free detection of optogenetically stimulated cellular activity by low coherence interferometry (Conference Presentation)", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902D (26 April 2016); https://doi.org/10.1117/12.2216404

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KEYWORDS

Interferometry

Optogenetics

Luminescence

Coherence (optics)

Electrophysiology

Microscopes

Phase measurement

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