Presentation + Paper
9 March 2016 Multi-modal in vivo imaging of brain blood oxygenation, blood flow and neural calcium dynamics during acute seizures
Dene Ringuette, Melanie A. Jeffrey, Peter L. Carlen M.D., Ofer Levi
Author Affiliations +
Abstract
Dysfunction of the vascular endothelium has been implicated in the development of epilepsy. To better understand the relation between vascular function and seizure and provide a foundation for interpreting results from functional imaging in chronic disease models, we investigate the relationship between intracellular calcium dynamics and local cerebral blood flow and blood oxygen saturation during acute seizure-like events and pharmacological seizure rescue. To probe the relation between the aforementioned physiological markers in an acute model of epilepsy in rats, we integrated three different optical modalities together with electrophysiological recordings: Laser speckle contrast imaging (LSCI) was used to study changes in flow speeds, Intrinsic optical signal imaging (IOSI) was used to monitor changes in oxygenated, de-oxygenated, and total hemoglobin concentration, and Calcium-sensitive dye imaging was used to monitor intracellular calcium dynamics. We designed a dedicated cortical flow chamber to remove superficial blood and dye resulting from the injection procedure, which reduced spurious artifacts. The near infrared light used for IOSI and LSCI was delivered via a light pipe integrated with the flow chamber to minimize the effect of fluid surface movement on illumination stability. Calcium-sensitive dye was injected via a glass electrode used for recording the local field potential. Our system allowed us to observe and correlate increases in intracellular calcium, blood flow and blood volume during seizure-like events and provide a quantitative analysis of neurovascular coupling changes associated with seizure rescue via injection of an anti-convulsive agent.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dene Ringuette, Melanie A. Jeffrey, Peter L. Carlen M.D., and Ofer Levi "Multi-modal in vivo imaging of brain blood oxygenation, blood flow and neural calcium dynamics during acute seizures", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 969011 (9 March 2016); https://doi.org/10.1117/12.2213962
Lens.org Logo
CITATIONS
Cited by 2 scholarly publications and 2 patents.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Blood

Stereolithography

Laser speckle contrast imaging

Calcium

Near infrared

Imaging systems

Epilepsy

RELATED CONTENT


Back to Top