CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 9568 > Article
Presentation
5 October 2015 Sensitive and selective real-time electrochemical monitoring of DNA repair (Presentation Recording)
Jason D. Slinker, Marc McWilliams, Fadwa Anka, Kenneth Balkus
Author Affiliations +
Proceedings Volume 9568, Organic Field-Effect Transistors XIV; and Organic Sensors and Bioelectronics VIII; 95681K (2015) https://doi.org/10.1117/12.2190226
Event: SPIE Organic Photonics + Electronics, 2015, San Diego, California, United States
Abstract
Unrepaired DNA damage can lead to mutation, cancer, and death of cells or organisms. However, due to the subtlety of DNA damage, it is difficult to sense the repair of damage products with high selectivity and sensitivity. Here, we show sensitive and selective electrochemical sensing of the repair activity of 8-oxoguanine and uracil glycosylases within DNA monolayers on gold by multiplexed analysis with silicon chips and low-cost electrospun nanofibers. Our approach involves comparing the electrochemical signal of redox probe modified monolayers containing the defect versus the rational control of defect-free monolayers. We find sequence-specific sensitivity thresholds on the order of femtomoles of proteins and dynamic ranges of over two orders of magnitude for each target. For 8-oxoguanine repair, temperature-dependent kinetics are extracted, showing exponential signal loss with time constants of seconds. Electrospun fibers are shown to behave similarly to conventional gold-on-silicon devices, showing the potential of these low-cost devices for sensing applications.
Conference Presentation
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Jason D. Slinker, Marc McWilliams, Fadwa Anka, and Kenneth Balkus "Sensitive and selective real-time electrochemical monitoring of DNA repair (Presentation Recording)", Proc. SPIE 9568, Organic Field-Effect Transistors XIV; and Organic Sensors and Bioelectronics VIII, 95681K (5 October 2015); https://doi.org/10.1117/12.2190226
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Cancer
Gold
Multiplexing
Nanofibers
Organisms
Proteins
Signal attenuation
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top