Semiconductor quantum dots as delivery and imaging platforms for intracellular assembly

Lauren D. Field; James B. Delehanty; Scott A. Walper; Kimihiro Susumu; Igor L. Medintz

doi:10.1117/12.2188392

21 August 2015 Semiconductor quantum dots as delivery and imaging platforms for intracellular assembly

Lauren D. Field, James B. Delehanty, Scott A. Walper, Kimihiro Susumu, Igor L. Medintz

Proceedings Volume 9550, Biosensing and Nanomedicine VIII; 955002 (2015) https://doi.org/10.1117/12.2188392
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Efficient and specific delivery of particles and drugs intracellularly is a critical area of research which can allow for further understanding of cellular processes and increased efficacy of therapeutic treatments. Visualizing these delivery mechanisms occurs through use of florescent molecules, some of which are bulky and can change the processes being studied. Alternatively, semiconductor nanocrystals called quantum dots (QDs) are nanoscale particles that provide a scaffold for drugs or targeting moieties while providing superior optical properties that include size-tunable photoluminescence and resistance to photobleaching. Utilizing these platforms, delivery methods and intracellular assembly can be studied. Potential delivery mechanisms include either specified delivery through attenuation to targeting ligands or systemic delivery through microinjection or electroporation. Once within the cytosol, the QDs can assemble to express proteins, a process that can be visualized through the use of FRET. The effectiveness of various delivery methods and QD surface chemistry has been analyzed and is described here.

Citation Download Citation

Lauren D. Field, James B. Delehanty, Scott A. Walper, Kimihiro Susumu, and Igor L. Medintz "Semiconductor quantum dots as delivery and imaging platforms for intracellular assembly", Proc. SPIE 9550, Biosensing and Nanomedicine VIII, 955002 (21 August 2015); https://doi.org/10.1117/12.2188392

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