A novel strategy to monitor microfluidic in-vitro blood-brain barrier models using impedance spectroscopy

J. Yeste; X. Illa; A. Guimerà; R. Villa

doi:10.1117/12.2180567

1 June 2015 A novel strategy to monitor microfluidic in-vitro blood-brain barrier models using impedance spectroscopy

J. Yeste, X. Illa, A. Guimerà, R. Villa

Proceedings Volume 9518, Bio-MEMS and Medical Microdevices II; 95180N (2015) https://doi.org/10.1117/12.2180567
Event: SPIE Microtechnologies, 2015, Barcelona, Spain

Abstract

In this work, we present the use of interdigitated electrodes (IDEs) for performing electrical impedance spectroscopy (EIS) measurements to monitor a microfluidic blood brain barrier model. In particular, an electrode configuration which would not impair the optical visualization of the cell culture is proposed. Numerical studies have been performed to evaluate the electrical impedance sensitivity of the proposed tetrapolar configuration along the cell barrier in a given microfluidic chamber geometry. The system has been validated using a home-made cyclo olefin polymer (COP) bioreactor and perforated poly (methyl methacrylate) (PMMA) sheets with different pore densities in order to simulate different cell barrier impedances.

Citation Download Citation

J. Yeste, X. Illa, A. Guimerà, and R. Villa "A novel strategy to monitor microfluidic in-vitro blood-brain barrier models using impedance spectroscopy", Proc. SPIE 9518, Bio-MEMS and Medical Microdevices II, 95180N (1 June 2015); https://doi.org/10.1117/12.2180567

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