Simultaneous detection of tissue autofluorescence decay distribution and time-gated photo-bleaching rates

Alexey Lihachev; Inesa Ferulova; Janis Spigulis; Mindaugas Tamosiunas

doi:10.1117/12.2178827

6 May 2015 Simultaneous detection of tissue autofluorescence decay distribution and time-gated photo-bleaching rates

Alexey Lihachev, Inesa Ferulova, Janis Spigulis, Mindaugas Tamosiunas

Proceedings Volume 9504, Photon Counting Applications 2015; 95040P (2015) https://doi.org/10.1117/12.2178827
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

Experimental methodology for parallel measurements of in-vivo skin autofluorescence (AF) lifetimes and photobleaching dynamic has been developed and tested. The AF lifetime decay distributions were periodically collected from fixed tissue area with subsequent detection of the fluorescence intensity decrease dynamic at different time gates after the pulse excitation. Temporal distributions of human in-vivo skin AF lifetimes and bleaching kinetics were collected and analyzed by means of commercial time-correlated single photon counting system.

Citation Download Citation

Alexey Lihachev, Inesa Ferulova, Janis Spigulis, and Mindaugas Tamosiunas "Simultaneous detection of tissue autofluorescence decay distribution and time-gated photo-bleaching rates", Proc. SPIE 9504, Photon Counting Applications 2015, 95040P (6 May 2015); https://doi.org/10.1117/12.2178827

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